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Qiagen 凯杰 Biosharp Omega

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UbcH13/Uev1A, active 250ug

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货号：23-051M

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Cascade Component
Enzyme Assays Human E2
Description
Catalogue Number 23-051
Brand Family Upstate
Trade Name
  • Upstate
Description UbcH13/Uev1A Protein, active, 10 µg
Alternate Names
  • UbcH13 - Ubiquitin-conjugating enzyme E2 N
  • Bendless-like ubiquitin-conjugating enzyme
  • Ubiquitin carrier protein N
  • UBE2N
  • Ubc13
  • Uev1A - Ubiquitin-conjugating enzyme E2 variant 1
  • CROC-1
  • UBE2V1
Background Information Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. Typically, a polyubiquitin chain that targets a protein for degradation by the proteasome is linked through lysine-48 (K48) of ubiquitin, however ubiquitin chains linked through other lysines such as K63 have also been found. K63 linked chains are non-degradative and play a role in signalling and protein trafficking. The heterodimeric E2 complex of Ubc13 and Uev1A has been shown to be involved in the formation of K63 linkages in conjunction with a number of E3 ligases including Parkin, CHIP and TRAF6.
References
Product Information
Applications
Application Active complex of two (2) full length, recombinant human enzymes: UbcH13 & Uev1A. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Cascade Component E2
Entrez Gene Number
Purification Method Purified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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