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Qiagen 凯杰 Biosharp Omega

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PRAK, unactive 250ug

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货号：14-335M

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-335
Brand Family Upstate
Trade Name
  • Upstate
Description PRAK Protein, unactive, 50 µg
Overview Recombinant full-length human PRAK with an N-terminal His-tag
References
Product Information
Applications
Application unactive, recombinant full-length human PRAK with an N-terminal His-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Source expressed in Sf9 cells
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.
Gene Symbol
  • MAPKAPK5
  • PRAK
Protein Target PRAK
Purification Method Ni-NTA agarose
Target Sub-Family CAMK
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q8IW41 # Mediates stress-induced small heat shock protein 27 phosphorylation.
SIZE: 473 amino acids; 54220 Da
SUBUNIT: Interacts with SQSTM1.
SUBCELLULAR LOCATION: Cytoplasm (Probable). Nucleus. Note=Also observed in the nucleus.
TISSUE SPECIFICITY: Expressed ubiquitously.
PTM: Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
SIMILARITY: SwissProt: Q8IW41 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight 54kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by phosphorylation of PRAK Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at -20C
Packaging Information
Material Size 1 mg
Transport Information
Supplemental Information
Specifications

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