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MRCK beta, active 250ug

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货号：14-643M

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-643
Brand Family Upstate
Trade Name
  • Upstate
Description MRCKβ Protein, active, 10 µg
Overview N-terminal His6-tagged, recombinant, human MRCKb amino acids 1-473
References
Product Information
Applications
Application Active, N-terminal His6-tagged, recombinant, human MRCKb amino acids 1-473, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Source human MRCKb amino acids 1-473, expressed by baculovirus in Sf21 cells
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization.
Gene Symbol
  • CDC42BPB
  • KIAA1124
  • MRCKB
Protein Target MRCKβ
Purification Method Ni2+/NTA-agarose
Target Sub-Family AGC
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9Y5S2 # May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).
COFACTOR: Magnesium (By similarity).
SIZE: 1711 amino acids; 194315 Da
SUBUNIT: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
SIMILARITY: SwissProt: Q9Y5S2 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 CNH domain. & Contains 1 CRIB domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
Molecular Weight 55.5kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 6 months at -70°C
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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