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Qiagen 凯杰 Biosharp Omega

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NLK, active 250ug

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货号：14-676M

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-676
Brand Family Upstate
Trade Name
  • Upstate
Description NLK Protein, active, 10 µg
Overview N-terminal 6His-tagged, recombinant, human NLK, amino acids 121-end
References
Product Information
Applications
Application Active, N-terminal 6His-tagged, recombinant, human NLK, amino acids 121-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Source expressed by baculovirus in Sf21 cells
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • NLK
  • DKFZp761G1211
  • FLJ21033
  • LAK1
Protein Target NLK
Purification Method Ni2+/NTA-agarose
Target Sub-Family CMGC
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9UBE8 # Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta- catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity).
COFACTOR: Magnesium (By similarity).
SIZE: 515 amino acids; 57048 Da
SUBUNIT: Interacts with HIPK2 and MYB.
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
PTM: Dually phosphorylated on Thr-291 and Tyr-293, which activates the enzyme (By similarity).
SIMILARITY: SwissProt: Q9UBE8 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight 48.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by phosphorylation of myelin basic protein (Catalog # 13-104)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at -70°C from date of shipment
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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