Anti-Calmodulin Binding Protein Epitope Tag, clone C16T (rabbit monoclonal IgG) - 炼石商城

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Anti-Calmodulin Binding Protein Epitope Tag, clone C16T (rabbit monoclonal IgG)

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货号：05-932

品牌：Millipore 密理博

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北京朝阳

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
Vrt	WB	Rb	Culture Supernatant	Monoclonal Antibody

Description
Catalogue Number	04-932
Replaces	05-932
Description	Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal
Alternate Names	calmodulin 1 calmodulin 1 (phosphorylase kinase, delta) phosphorylase kinase, delta subunit
Background Information	Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.

References

Product Information
Format	Culture Supernatant
Control	Samples containing the calmodulin binding protein epitope tag.
Presentation	Cultured supernatant with 0.05% sodium azide.

Applications
Application	This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.
Key Applications	Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.
Epitope	CBP epitope
Clone	C16T
Host	Rabbit
Specificity	Recombinant proteins containing the calmodulin binding protein epitope tag.
Isotype	IgG
Species Reactivity	Vertebrates
Antibody Type	Monoclonal Antibody
Entrez Gene Summary	Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]
Gene Symbol	CALM CALML2 CAM CAM1 CAM2 CAM3 CAMB CAMC CAMI CAMIII CaM DD132 PHKD
UniProt Number	P62158
UniProt Summary	FUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. SUBUNIT STRUCTURE: Interacts with MYO1C and RRAD By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. PTM: Ubiquitination results in a strongly decreased activity By similarity.Phosphorylation results in a decreased activity By similarity. MISCELLANEOUS: This protein has four functional calcium-binding sites. SEQUENCE SIMILARITIES: Belongs to the calmodulin family. Contains 4 EF-hand domains.
Molecular Weight	The molecular weight is related to the tagged protein.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on lysates from transformed E. coli. Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µl

Transport Information

Supplemental Information

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