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Anti-PAK1 (rabbit polyclonal)

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货号：07-1451

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Mk WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-1451
Replaces 04-584; 04-582
Description Anti-PAK1 Antibody
Alternate Names
  • STE20 homolog, yeast
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • p21-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Background Information p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).
References
Product Information
Format Affinity Purified
Control
  • NIH/3T3 cell lysate
Presentation Purified rabbit polyclonal liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.
Applications
Application Anti-PAK1 Antibody is an antibody against PAK1 for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen KLH-conjugated synthetic peptide corresponding to the N-terminus of human PAK1.
Epitope N-terminus
Host Rabbit
Specificity This antibody recognizes the N-terminus of PAK1 but does not cross-react with PAK2, PAK3, or other PAKs.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • Alpha-PAK
  • EC 2.7.11.1
  • MGC130000
  • MGC130001
  • PAK-1
  • PAKalpha
  • p65-PAK
Purification Method Affinity Purfied
UniProt Number
UniProt Summary FUNCTION: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation By similarity.
SUBUNIT STRUCTURE: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. Interacts with ARHGEF7. Also interacts with CRIPAK. Interacts with NISCH By similarity. Interacts with SCRIB.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction › focal adhesion. Note: Recruited to focal adhesions upon activation.
PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Molecular Weight Approx. 62 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected PAK-1 on 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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