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Anti-Sp1 (rabbit polyclonal)

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号:ABE135

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP, ICC Rb Purified Polyclonal Antibody
Description
Catalogue Number ABE135
Replaces MABE550
Description Anti-Sp1 Antibody
Alternate Names
  • Sp1 transcription factor
  • specificity protein 1
Background Information Sp1 was one of the first transcription factors to be characterized in mammalian cells. The Sp1 protein plays a role in the regulation of gene expression. This protein has three Cys2His2 zinc-finger motifs that have been shown to bind to the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' also known as a GC box. Sp1 is a ubiquitous transcription factor required for the expression of a variety of genes, such as those involved in the cell cycle and development. Interaction of the Sp1 protein with the GC box activates transcription of specific mRNAs from genes that contain functional recognition sites.
References
Product Information
Format Purified
Control
  • HeLa nuclear extract
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application Anti-Sp1 Antibody is a Rabbit Polyclonal Antibody for detection of Sp1 also known as Sp1 transcription factor, specificity protein 1 & has been validated in WB, IP, ICC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Sp1 in HeLa and A431 cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to full-length human Sp1.
Epitope N/A
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes Sp1.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Demonstrated to react with human, mouse, and rat. Homology to chimpanzee, canine, and rhesus monkey is 99%.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TSFP1
  • SP1
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.

SIZE: 785 amino acids; 80693 Da

SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.

SUBCELLULAR LOCATION: Nucleus.

PTM: O-glycosylated; contains N-acetylglucosamine side chains.

SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
Molecular Weight 95 kDa and 105 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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