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Anti-Plet-1, clone 1D4 (rat monoclonal)

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货号：MAB4416

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, IHC, FC R Purified Monoclonal Antibody
Description
Catalogue Number MAB4416
Description Anti-Plet-1 (Placenta-expressed transcript 1 protein) Antibody, clone 1D4
Alternate Names
  • chromosome 11 open reading frame 34
  • hypothetical protein LOC349633
  • placenta expressed transcript 1
Background Information Placenta-expressed transcript-1 (Plet-1) is a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Its observation has been restricted to developing mesonephros and pharyngeal endoderm. Observation in adult tissue is more widely expressed, having been found in several locations such as mammary, pancreas and prostate epithelia. Its location in the pancreas is notable as the pancreas is a well known reserve for stem cells and progenitor cells. Within the pancreas, Plet-1 has been shown to be localized in the major duct epithelium, and as such will serve as an excellent marker for epithelial progenitor cells and stem cells, and as a useful implement for observing the genetics of linear relationships and the operation of molecular mechanisms for homeostasis, development and wound healing in organ and tissue systems.
Recently, Plet-1 has been identified as a specific marker of early thymic epithelial progenitor cells. In the postgastrulation embryo, Plet-1 expression is highly restricted to the developing pharyngeal endoderm and mesonephros. This antibody recognizes the same protein as MTS20 and MTS24.
References
Product Information
Format Purified
Control
  • Human placenta cell lysate
Presentation Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application This Anti-Plet-1 (Placenta-expressed transcript 1 protein) Antibody, clone 1D4 is validated for use in WB, IC, IH, FC for the detection of Plet-1 (Placenta-expressed transcript 1 protein).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Flow Cytometry
Application Notes Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Depreter, M.G.L., et al., 2008).

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Depreter, M.G.L., et al., 2008).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Depreter, M.G.L., et al., 2008).
Biological Information
Immunogen Full length recombinant protein corresponding to Plet-1.
Epitope Unknown
Clone 1D4
Host Rat
Specificity This antibody recognizes Plet-1.
Isotype IgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Demonstrated to react with human.
Predicted to react with mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PLET1
  • C11orf34
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: May participate in the wound response during the healing process, and promote wound repair.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential.
Molecular Weight ~ 23 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in human placenta cell lysate.

Western Blot Analysis: A 1:2,500 dilution of this antibody detected Plet-1 in 10 µg of human placenta cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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