Anti-ATR (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Ca, Eq, B	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Ca, Eq, B

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-070
Description	Anti-ATR Antibody
Alternate Names	Ataxia telangiectasia and Rad3-related protein FRAP-related protein 1 FRAP-related protein-1 MEC1 mitosis entry checkpoint 1
Background Information	ATR (Ataxia Telangiectasia and Rad3-related kinase) is a protein kinase that regulates cell cycle checkpoints and DNA repair. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1). ATR belongs to the PI3/PI4-kinase family, and is most closely related to ATM (Ataxia Telangiectasia Mutated kinase).

Description

Catalogue Number

09-070

Description

Anti-ATR Antibody

Alternate Names

Ataxia telangiectasia and Rad3-related protein
FRAP-related protein 1
FRAP-related protein-1
MEC1
mitosis entry checkpoint 1

Background Information

ATR (Ataxia Telangiectasia and Rad3-related kinase) is a protein kinase that regulates cell cycle checkpoints and DNA repair. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1). ATR belongs to the PI3/PI4-kinase family, and is most closely related to ATM (Ataxia Telangiectasia Mutated kinase).

Product Information
Format	Affinity Purified
Control	HeLa cell lysate
Presentation	Purified rabbit serum in 0.1M Tris-Glycine (pH7.4) and 150mM NaCl containing 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

HeLa cell lysate

Presentation

Purified rabbit serum in 0.1M Tris-Glycine (pH7.4) and 150mM NaCl containing 0.05% sodium azide.

Applications
Application	Anti-ATR Antibody detects level of ATR & has been published & validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot (SNAP ID) Analysis: A 1:500 dilution from a previous lot of this antibody was used to was detect ATR in HeLa, NIH3T3, and C6 cell lysates.

Applications

Application

Anti-ATR Antibody detects level of ATR & has been published & validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blot (SNAP ID) Analysis: A 1:500 dilution from a previous lot of this antibody was used to was detect ATR in HeLa, NIH3T3, and C6 cell lysates.

Biological Information
Immunogen	KLH linear peptide corresponding to amino acids 1887-1902 of ATR.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes ATR.
Species Reactivity	Human Mouse Rat Canine Horse Bovine
Species Reactivity Note	Proven to react with human, mouse, and rat. Predicted to react with dog, horse, and cow based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001175
Entrez Gene Summary	The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.
Gene Symbol	EC 2.7.11.1 FRP1 MEC1 SCKL SCKL1
Purification Method	Affinity Purfied
UniProt Number	Q13535
UniProt Summary	FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Manganese. ENZYME REGULATION: Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine, wortmannin and LY294002. SUBUNIT STRUCTURE: Forms an heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164. SUBCELLULAR LOCATION: Nucleus. Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling. TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. PTM: Phosphorylated; autophosphorylates in vitro. INVOLVEMENT IN DISEASE: Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 2 HEAT repeats. Contains 1 PI3K/PI4K domain.
Molecular Weight	250 kDa

Biological Information

Immunogen

KLH linear peptide corresponding to amino acids 1887-1902 of ATR.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes ATR.

Species Reactivity

Human
Mouse
Rat
Canine
Horse
Bovine

Species Reactivity Note

Proven to react with human, mouse, and rat. Predicted to react with dog, horse, and cow based on sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001175

Entrez Gene Summary

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.

Gene Symbol

EC 2.7.11.1
FRP1
MEC1
SCKL
SCKL1

Purification Method

Affinity Purfied

UniProt Number

Q13535

UniProt Summary

FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Manganese.
ENZYME REGULATION: Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine, wortmannin and LY294002.
SUBUNIT STRUCTURE: Forms an heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164.
SUBCELLULAR LOCATION: Nucleus. Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
TISSUE SPECIFICITY: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
PTM: Phosphorylated; autophosphorylates in vitro.
INVOLVEMENT IN DISEASE: Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.

Molecular Weight

250 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on HeLa cell lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect ATR in HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect ATR in HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-ATR (rabbit polyclonal)

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