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Milli-Mark™ Anti-CD25-FITC clone ACT-1

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货号：FCMAB189F

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC M FITC Monoclonal Antibody
Description
Catalogue Number FCMAB189F
Trade Name
  • Milli-Mark
Description Milli-Mark™ Anti-CD25-FITC Antibody, clone ACT-1
Alternate Names
  • Interleukin-2 receptor subunit alpha
  • IL-2 receptor subunit alpha
  • IL-2-RA
  • p55
  • TAC antigen
Background Information The interleukin-2 receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2 (Ishii, 1995). The 55 kDa α-chain is called CD25. CD25 is strongly expressed on the surface of activated T cells (Uchiyama, 1981), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide (Sugamura, 1997). In normal peripheral blood a variable expression on lymphoid cells (about 3-25%) is observed. The specificity of Anti-CD25, ACT-1, is identical to that of CD25-clustered antibodies (Schwarting, 1987; Aziz, 1996).
References
Product Information
Format FITC
Control
  • Human Lymphocytes
Presentation Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Applications
Application This Milli-Mark Anti-CD25-FITC Antibody, clone ACT-1 is validated for use in FC for the detection of CD25.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen Phytohemagglutinin-stimulated blood lymphocytes
Clone ACT-1
Host Mouse
Specificity Antibody recognizes Human CD25 antigen.
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Purification Method Protein A Purfied
UniProt Number
Molecular Weight 55 kDa (Uchiyama, 1981)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by flow cytometry using human lymphocytes
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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