Anti-phospho-c-Myc (Thr58/Ser62), clone E203 (rabbit monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IP	M	Culture Supernatant	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, IP

Culture Supernatant

Monoclonal Antibody

Description
Catalogue Number	05-217
Replaces	04-403
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-PI3 Kinase Antibody, p85, N-SH2,
Alternate Names	PI3-kinase p110 subunit alpha PtdIns-3-kinase p110 phosphatidylinositol 3-kinase, catalytic 110-KD alpha phosphatidylinositol 3-kinase, catalytic alpha polypeptide phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform phosphoinositide-3-kinase, catalytic, alpha polypeptide
Background Information	The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signalling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.

Description

Catalogue Number

05-217

Replaces

04-403

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-PI3 Kinase Antibody, p85, N-SH2,

Alternate Names

PI3-kinase p110 subunit alpha
PtdIns-3-kinase p110
phosphatidylinositol 3-kinase, catalytic
110-KD
alpha
phosphatidylinositol 3-kinase, catalytic
alpha polypeptide
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Background Information

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signalling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.

Product Information
Format	Culture Supernatant
Control	Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Included Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation	Mouse monoclonal IgG in buffer containing culture supernatant fraction in frozen solution.

Product Information

Format

Culture Supernatant

Control

Positive Control Included: Non-Stim A431 Cell Lysate (12-301).
Included Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Presentation

Mouse monoclonal IgG in buffer containing culture supernatant fraction in frozen solution.

Applications
Application	Anti-PI3 Kinase Antibody, p85, N-SH2, is an antibody against PI3 Kinase for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation: Use 5 µL per sample.

Applications

Application

Anti-PI3 Kinase Antibody, p85, N-SH2, is an antibody against PI3 Kinase for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation: Use 5 µL per sample.

Biological Information
Immunogen	GST fusion protein corresponding to N-Terminal SH2 region (amino acid residues 327-434) of rat phosphoinositide 3-Kinase.
Epitope	N-terminus
Clone	clone UB93-3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes N-SH2 region of p85 alpha subunit of PI3 Kinase.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, rat; mouse; other species cross-reactivity is unknown.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_852556
Entrez Gene Summary	Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
Gene Symbol	PIK3R1 GRB1 PI3K p85-ALPHA
Purification Method	Unpurified
UniProt Number	P27986
UniProt Summary	FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. SIZE: 724 amino acids; 83598 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1. TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance. SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.
Molecular Weight	85 kDa

Biological Information

Immunogen

GST fusion protein corresponding to N-Terminal SH2 region (amino acid residues 327-434) of rat phosphoinositide 3-Kinase.

Epitope

N-terminus

Clone

clone UB93-3

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes N-SH2 region of p85 alpha subunit of PI3 Kinase.

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human, rat; mouse; other species cross-reactivity is unknown.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_852556

Entrez Gene Summary

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

Gene Symbol

PIK3R1
GRB1
PI3K
p85-ALPHA

Purification Method

Unpurified

UniProt Number

P27986

UniProt Summary

FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
SIZE: 724 amino acids; 83598 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1.
TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance.
SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.

Molecular Weight

85 kDa

Product Usage Statements
Quality Assurance	Western blot Analysis: A 1:100-1:200 dilution of this antibody detected PI3-Kinase in RIPA lysates from human A431 carcinoma cells and previously from mouse 3T3/A31 fibroblasts.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Western blot Analysis: A 1:100-1:200 dilution of this antibody detected PI3-Kinase in RIPA lysates from human A431 carcinoma cells and previously from mouse 3T3/A31 fibroblasts.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at from date of receipt.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-c-Myc (Thr58/Ser62), clone E203 (rabbit monoclonal)

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