Anti-NK1.1 (mouse), clone PK136, Azide Free (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	FC, ACT, IP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

FC, ACT, IP

Purified

Monoclonal Antibody

Description
Catalogue Number	MABF1495Z
Description	Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free
Alternate Names	Killer cell lectin-like receptor subfamily B member 1B allele A Killer cell lectin-like receptor subfamily B member 1C CD161b CD161c CD161 antigen-like family member B CD161 antigen-like family member C Ly-55b Ly-55c Lymphocyte antigen 55b Lymphocyte antigen 55c Natural killer cell surface protein NKR-P1B allele SJL/BALB Natural killer cell surface protein P1-40 NKR-P1 34 NK1.1 NKR-P1.9 NKR-P1 40 NKR-P1B NKR-P1C
Background Information	The NK cell receptor protein 1 (NKR-P1), a.k.a. CD161, molecules represent a family of type II transmembrane C-type lectin-like receptors expressed predominantly by NK cells. A total of five murine NKR-P1-coding genes (Klrb1a/b/c/d/f) have been identified, with NKR-P1A/C/F (CD161a/c/f) functioning as stimulatory receptors that possess a charged transmembrane arginine esidue thought to be important for association with the FcRγ adaptor protein, and NKR-P1B/D as two inhibitory receptors that possess a consensus cytoplasmic ITIM (L/VxYxxL/I/V) motif. The ITIM motif is shown to mediate NKR-P1B recruitment of Src homology 2 (SH2)-containing protein tyrosine phosphatase-1 (SHP-1) in a phosphorylation-dependent manner. All murine NKR-P1 proteins possess a Cys-X-Cys-Pro (CxCP) motif that mediates association with the Src-related nonreceptor protein tyrosine kinase p56lck. Both NKR-P1B and NKR-P1C functionally associate with p56lck, while mutation of the CxCP motif abolishes NKR-P1B/C-mediated signal transduction. Likewise, mutation of ITIM motif abolishes NKR-P1B SHP-1 association as well as NKR-P1B-dependent inhibitory signaling.

Description

Catalogue Number

MABF1495Z

Description

Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free

Alternate Names

Killer cell lectin-like receptor subfamily B member 1B allele A
Killer cell lectin-like receptor subfamily B member 1C
CD161b
CD161c
CD161 antigen-like family member B
CD161 antigen-like family member C
Ly-55b
Ly-55c
Lymphocyte antigen 55b
Lymphocyte antigen 55c
Natural killer cell surface protein NKR-P1B allele SJL/BALB
Natural killer cell surface protein P1-40
NKR-P1 34
NK1.1
NKR-P1.9
NKR-P1 40
NKR-P1B
NKR-P1C

Background Information

The NK cell receptor protein 1 (NKR-P1), a.k.a. CD161, molecules represent a family of type II transmembrane C-type lectin-like receptors expressed predominantly by NK cells. A total of five murine NKR-P1-coding genes (Klrb1a/b/c/d/f) have been identified, with NKR-P1A/C/F (CD161a/c/f) functioning as stimulatory receptors that possess a charged transmembrane arginine esidue thought to be important for association with the FcRγ adaptor protein, and NKR-P1B/D as two inhibitory receptors that possess a consensus cytoplasmic ITIM (L/VxYxxL/I/V) motif. The ITIM motif is shown to mediate NKR-P1B recruitment of Src homology 2 (SH2)-containing protein tyrosine phosphatase-1 (SHP-1) in a phosphorylation-dependent manner. All murine NKR-P1 proteins possess a Cys-X-Cys-Pro (CxCP) motif that mediates association with the Src-related nonreceptor protein tyrosine kinase p56lck. Both NKR-P1B and NKR-P1C functionally associate with p56lck, while mutation of the CxCP motif abolishes NKR-P1B/C-mediated signal transduction. Likewise, mutation of ITIM motif abolishes NKR-P1B SHP-1 association as well as NKR-P1B-dependent inhibitory signaling.

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Product Information

Format

Purified

Presentation

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Applications
Application	Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free is an antibody against NK1.1 (mouse) for use in Flow Cytometry, Activity Assay, Immunohistochemistry, Immunoprecipitation.
Key Applications	Flow Cytometry Activity Assay Immunoprecipitation
Application Notes	Activity Assay: Representative lots, when administered to mice via i.p. injection, depleted splenic natural killer (NK) cell activity in a dose-dependent manner (Xiao, T.Z., et al. (2012). Biol. Blood Marrow Transplant. 18(2):200-209; Seaman, W.E., et al. (1987). J. Immunol. 138(12):4539-4544). Activity Assay: A representative lot activated target cell killing by cross-linking NK cell surface NK1.1 (NKR-P1C) and target cell surface Fc receptor. Ab-induced redirected lysis (AIRL) assay employing NK cells expressing both NKR-P1B and NKR-P1C prevented NK cell cytotoxicity activation by clone PK136 (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SHP-1, but not SHP-2 or SHIP, from Swiss NIH mouse-derived MNK-1 pre-NK cells upon upregulating NKR-P1B intracellular ITIM motif tyrosine phosphorylation by pervanadate treatment (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Flow Cytometry Analysis: A representative lot immunostained the surface of Jurkat transfectants expressing C57BL/6J (B6) mouse-derived NKR-P1B/CD161b or Swiss NIH (Sw) mouse-derived NKR-P1C/CD161c/NK1.1, but not Jurkat transfectants expressing B6-derived NKR-P1A/CD161a (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Flow Cytometry Analysis: A representative lot immunostained NKR-P1B/CD161b-expressing NK cells from Swiss NIH (Sw) mouse strain, NKR-P1C/CD161c/NK1.1-expressing NK cells from C57BL/6J (B6), as well as (B6 3 Sw)F1 cross-strain-derived NK cells expressing both NKR-P1B and NKR-P1C (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Note: Clone PK136 is also available in the following conjugated forms for flow cytometry application, APC (MABF1487 & MABF1488), FITC (MABF1489 & MABF1490), PE (MABF1491), PerCP-Cy5.5 (MABF1493 & MABF1494). redFluor™ 710 (MABF1496 & MABF1497).

Applications

Application

Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free is an antibody against NK1.1 (mouse) for use in Flow Cytometry, Activity Assay, Immunohistochemistry, Immunoprecipitation.

Key Applications

Flow Cytometry
Activity Assay
Immunoprecipitation

Application Notes

Activity Assay: Representative lots, when administered to mice via i.p. injection, depleted splenic natural killer (NK) cell activity in a dose-dependent manner (Xiao, T.Z., et al. (2012). Biol. Blood Marrow Transplant. 18(2):200-209; Seaman, W.E., et al. (1987). J. Immunol. 138(12):4539-4544).
Activity Assay: A representative lot activated target cell killing by cross-linking NK cell surface NK1.1 (NKR-P1C) and target cell surface Fc receptor. Ab-induced redirected lysis (AIRL) assay employing NK cells expressing both NKR-P1B and NKR-P1C prevented NK cell cytotoxicity activation by clone PK136 (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SHP-1, but not SHP-2 or SHIP, from Swiss NIH mouse-derived MNK-1 pre-NK cells upon upregulating NKR-P1B intracellular ITIM motif tyrosine phosphorylation by pervanadate treatment (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923).
Flow Cytometry Analysis: A representative lot immunostained the surface of Jurkat transfectants expressing C57BL/6J (B6) mouse-derived NKR-P1B/CD161b or Swiss NIH (Sw) mouse-derived NKR-P1C/CD161c/NK1.1, but not Jurkat transfectants expressing B6-derived NKR-P1A/CD161a (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923).
Flow Cytometry Analysis: A representative lot immunostained NKR-P1B/CD161b-expressing NK cells from Swiss NIH (Sw) mouse strain, NKR-P1C/CD161c/NK1.1-expressing NK cells from C57BL/6J (B6), as well as (B6 3 Sw)F1 cross-strain-derived NK cells expressing both NKR-P1B and NKR-P1C (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923).
Note: Clone PK136 is also available in the following conjugated forms for flow cytometry application, APC (MABF1487 & MABF1488), FITC (MABF1489 & MABF1490), PE (MABF1491), PerCP-Cy5.5 (MABF1493 & MABF1494). redFluor™ 710 (MABF1496 & MABF1497).

Biological Information
Immunogen	Mouse spleen and bone marrow cells (Koo, G.C., and Peppard, J.R. (1984). Hybridoma. 3(3):301-303).
Epitope	Extracellular domain.
Clone	PK136
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone PK136 recognizes murine NKR-P1B/CD161b (UniProt P27812) and NK1.1/NKR-P1C/CD161c (UniProt P27814), but not CD161a/NKR-P1A (P27811). Upon activation by PK136 binding, NKR-P1C/NK1.1 signals to activate, while NKR-P1B signals to suppress, NK cells for cytotoxic killing of target cells (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Epitope is present in both spliced isoforms of murine NK1.1/CD161c reported by UniProt (P27814). Clone PK136 does not react with CD161a (by Klrb1a gene) or C57BL/6 mouse strain-derived CDC161b variant (by Klrb1b gene, also known as Klrb1d). There exists a fifth murine CD161 gene Klrb1f, reactivity of this mAb toward this gene product has not been established.
Isotype	IgG2aκ
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	ABB72026 NP_001153376
Gene Symbol	Klrb1b Ly55b Nkrp1b Klrb1c Ly55c Nkrp1c
Purification Method	Protein G purified.
UniProt Number	P27812 P27814
Molecular Weight	25.08 kDa (NK1.1/CD161c isoform 1) and 24.77 kDa (NK1.1/CD161c isoform 2) calculated.

Biological Information

Immunogen

Mouse spleen and bone marrow cells (Koo, G.C., and Peppard, J.R. (1984). Hybridoma. 3(3):301-303).

Epitope

Extracellular domain.

Clone

PK136

Concentration

Please refer to lot specific datasheet.

Host

Mouse

Specificity

Clone PK136 recognizes murine NKR-P1B/CD161b (UniProt P27812) and NK1.1/NKR-P1C/CD161c (UniProt P27814), but not CD161a/NKR-P1A (P27811). Upon activation by PK136 binding, NKR-P1C/NK1.1 signals to activate, while NKR-P1B signals to suppress, NK cells for cytotoxic killing of target cells (Carlyle, J.R., et al. (1999). J. Immunol. 162(10):5917-5923). Epitope is present in both spliced isoforms of murine NK1.1/CD161c reported by UniProt (P27814). Clone PK136 does not react with CD161a (by Klrb1a gene) or C57BL/6 mouse strain-derived CDC161b variant (by Klrb1b gene, also known as Klrb1d). There exists a fifth murine CD161 gene Klrb1f, reactivity of this mAb toward this gene product has not been established.

Isotype

IgG2aκ

Species Reactivity

Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

Gene Symbol

Klrb1b
Ly55b
Nkrp1b
Klrb1c
Ly55c
Nkrp1c

Purification Method

Protein G purified.

UniProt Number

Molecular Weight

25.08 kDa (NK1.1/CD161c isoform 1) and 24.77 kDa (NK1.1/CD161c isoform 2) calculated.

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry in mouse splenocytes. Flow Cytometry Analysis: 1.0 µg of this antibody detected NK1.1-positive mouse splenocytes.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected NK1.1-positive mouse splenocytes.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 μg

Packaging Information

Material Size

100 μg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-NK1.1 (mouse), clone PK136, Azide Free (mouse monoclonal)

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