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Anti-Orai1, Intracellular

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货号：AB9868

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB9868
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Orai1 Antibody, Intracellular
Background Information TRP (transient receptor potential)-related ion channels (TRPCs) have been postulated as candidates for forming both receptor-operated (ROCE) and capacitative Ca2+ entry channels across the plasma membrane. ER transmembrane protein STIM1 senses the depletion of Ca2+ from ER stores. Orai, a regulatory β-subunit of the TRPC ion channel α-subunit, transduces the STIM1 signal into activation of the TRPC. Thus, Orai proteins by interacting with TRPCs act as regulatory subunits that confer STIM1-mediated store depletion sensitivity to these channels and regulate Ca2+ influx through the plasma membrane.
References
Product Information
Format Purified
Presentation Affinity purified immunoglobulin in PBS with 0.1% NaN3
Applications
Application This Anti-Orai1 Antibody, Intracellular is validated for use in WB for the detection of Orai1.
Key Applications
  • Western Blotting
Application Notes Immunoblotting: 1:1,000-1:5,000
Immunofluorescence: 1:1,000-1:2,500
Immunoprecipitation: 1 µg
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic Peptide from the Intracellular domain of Orai1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity ORai1, intracellular. Detects both the glycosylated and unglycosylated forms of ORai1. By Western blot the antibody reacts with the ~ 50 kDa ORai1 protein. Additional bands between 35 and 50 kDa may be seen if analyzing overexpressed ORai1. These additional bands most likely represent different degrees of glycosylation of ORai1
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Gene Symbol
  • ORAI1
  • CRACM1
  • TMEM142A
UniProt Number
Molecular Weight 50 Kda
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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