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Anti-phospho-Flt-1, (Tyr1213)

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货号：07-758

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Gt, H, M, R, Sh WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-758
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Flt-1 (Tyr1213) Antibody
Alternate Names
  • Vascular endothelial growth factor receptor 1 (VEGFR-1)
  • Vascular permeability factor receptor
  • Tyrosine-protein kinase FRT
  • Fms-like tyrosine kinase
References
Product Information
Format Affinity Purified
Control
  • Recombinant Flt-1 (Catalogue No. 14-562) phosphorylated by ATP
Presentation Immunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Applications
Application Anti-phospho-Flt-1 (Tyr1213) Antibody detects level of phospho-Flt-1 (Tyr1213) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen KLH-conjugated, synthetic peptide containing the sequence (...VR[pY]VN...) in which [pY] corresponds to phospho-tyrosine at residue 1213 of human Flt-1. The immunizing sequence is identical in marmoset, has 14/15 identical amino acids in rat, goat, sheep, cow, and rhesus monkey and 13/15 identical amino acids in mouse.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes phospho-Flt-1 (Tyr1213).
Isotype IgG
Species Reactivity
  • Bovine
  • Goat
  • Human
  • Mouse
  • Rat
  • Sheep
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM]
Gene Symbol
  • FLT1
  • VEGFR1
  • FLT
  • FRT
  • Flt-1
  • VEGFR-1
Modifications
  • Phosphorylation
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P17948 # Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
SIZE: 1338 amino acids; 150769 Da
SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.
SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted.
TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight ~180 kDa (66 kDa for the fragment tested in QC)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot on recombinant Flt-1 (Catalog # 14-562) phosphorylated upon incubation with ATP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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