Anti-Substance P Receptor (guinea pig polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, IF, IHC, WB	Gp	Unpurified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ICC, IF, IHC, WB

Unpurified

Polyclonal Antibody

Description
Catalogue Number	AB15810
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Substance P Receptor Antibody
Alternate Names	Substance-P receptor Neurokinin-1 receptor NK-1 receptor NK-1R SPR Tachykinin receptor 1
Background Information	Substance-P receptor (UniProt P14600; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the Tacr1 (also known as Nk1r, Tac1r) gene (Gene ID 24807) in rat species. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addition, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, the NK-1R interacts with multiple G proteins, including Gαs, Gαq/11, Gαi/o, Gα12, and Gα13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exist a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.

Description

Catalogue Number

AB15810

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Substance P Receptor Antibody

Alternate Names

Substance-P receptor
Neurokinin-1 receptor
NK-1 receptor
NK-1R
SPR
Tachykinin receptor 1

Background Information

Substance-P receptor (UniProt P14600; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the Tacr1 (also known as Nk1r, Tac1r) gene (Gene ID 24807) in rat species. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addition, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, the NK-1R interacts with multiple G proteins, including Gαs, Gαq/11, Gαi/o, Gα12, and Gα13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exist a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.

Product Information
Format	Unpurified
Control	Rat brain tissue lysate
Presentation	Guinea Pig polyclonal antibody serum with 0.05% sodium azide

Product Information

Format

Unpurified

Control

Rat brain tissue lysate

Presentation

Guinea Pig polyclonal antibody serum with 0.05% sodium azide

Applications
Application	This Guinea pig polyclonal Anti-Substance P Receptor Antibody, Cat. No. AB15810 detects levels of Substance P receptor, and has been published and validated for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications	Immunocytochemistry Immunofluorescence Immunohistochemistry Western Blotting
Application Notes	Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected substance P receptor in 10 µg of human brain tissue lysate. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected substance P receptor in rat brain tissue sections. Immunofluorescence Analysis: A 1:5,000-10,000 dilution from a representative lot detected substance-P receptor in frozen rat spinal cord tissue sections (25 µm cryostat) fixed with 4% paraformaldehyde containing 0.18% picric acid by indirect immunofluorescence staining using a rhodamine Red-X-conjugated secondary antibody. Immunofluorescence Analysis: A representative lot detected substance-P receptor- (NK-1R) positive neurons by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed and 0.2% Triton X-100-permeabilized mouse brain cryosections (Manzke, T., et la. (2010). J. Clin. Invest. 120(11):4118-4128). Immunocytochemistry Analysis: A representative lot detected substance-P receptor (NK-1R) by fluorescent immunocytochemistry staining of cultured primary E17-E18 rat embryonic amygdala neurons fixed with 4% paraformaldehyde and permeabilized with 0.1% saponin (Yu, Y.J., et al. (2009). J. Neurosci. 29(1):222-233).

Applications

Application

This Guinea pig polyclonal Anti-Substance P Receptor Antibody, Cat. No. AB15810 detects levels of Substance P receptor, and has been published and validated for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Key Applications

Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blotting

Application Notes

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected substance P receptor in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected substance P receptor in rat brain tissue sections.

Immunofluorescence Analysis: A 1:5,000-10,000 dilution from a representative lot detected substance-P receptor in frozen rat spinal cord tissue sections (25 µm cryostat) fixed with 4% paraformaldehyde containing 0.18% picric acid by indirect immunofluorescence staining using a rhodamine Red-X-conjugated secondary antibody.

Immunofluorescence Analysis: A representative lot detected substance-P receptor- (NK-1R) positive neurons by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed and 0.2% Triton X-100-permeabilized mouse brain cryosections (Manzke, T., et la. (2010). J. Clin. Invest. 120(11):4118-4128).

Immunocytochemistry Analysis: A representative lot detected substance-P receptor (NK-1R) by fluorescent immunocytochemistry staining of cultured primary E17-E18 rat embryonic amygdala neurons fixed with 4% paraformaldehyde and permeabilized with 0.1% saponin (Yu, Y.J., et al. (2009). J. Neurosci. 29(1):222-233).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the C-terminal end sequence of rat substance P receptor.
Epitope	C-Terminus
Host	Guinea Pig
Specificity	This guinea pig polyclonal antiserum targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_036799
Gene Symbol	Tacr1 Tac1r Nk1r
Purification Method	Unpurified.
UniProt Number	P14600
Molecular Weight	~58/54 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of rat substance P receptor.

Epitope

C-Terminus

Host

Guinea Pig

Specificity

This guinea pig polyclonal antiserum targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_036799

Gene Symbol

Tacr1
Tac1r
Nk1r

Purification Method

Unpurified.

UniProt Number

P14600

Molecular Weight

~58/54 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: A 1:5000 dilution from a representative lot detected substance-P receptor in 10 µg of rat brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:5000 dilution from a representative lot detected substance-P receptor in 10 µg of rat brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µL

Packaging Information

Material Size

50 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Substance P Receptor (guinea pig polyclonal)

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