Anti-mPER1 (residues 6-21) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, R	WB	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H, R

Serum

Polyclonal Antibody

Description
Catalogue Number	AB2201
Description	Anti-mPER1 (residues 6-21) Antibody
Alternate Names	Circadian clock protein PERIOD 1 Circadian pacemaker protein Rigui Period, drosophila homolog of period 1 period (Drosophila) homolog 1 period homolog 1 (Drosophila)
Background Information	This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.

Description

Catalogue Number

AB2201

Description

Anti-mPER1 (residues 6-21) Antibody

Alternate Names

Circadian clock protein PERIOD 1
Circadian pacemaker protein Rigui
Period, drosophila
homolog of period 1
period (Drosophila) homolog 1
period homolog 1 (Drosophila)

Background Information

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.

Product Information
Format	Serum
Control	Human brain lysate
Presentation	Serum with 0.05% NaN3

Product Information

Format

Serum

Control

Human brain lysate

Presentation

Serum with 0.05% NaN3

Applications
Application	Detect mPER1 (residues 6-21) using this Anti-mPER1 (residues 6-21) Antibody validated for use in WB.
Key Applications	Western Blotting
Application Notes	Immunohistichemistry: Tested on whole brain (SCN; peak staining at CT/ZT12). Optimium working dilutions need to be determined by end used.

Applications

Application

Detect mPER1 (residues 6-21) using this Anti-mPER1 (residues 6-21) Antibody validated for use in WB.

Key Applications

Western Blotting

Application Notes

Immunohistichemistry:
Tested on whole brain (SCN; peak staining at CT/ZT12). Optimium working dilutions need to be determined by end used.

Biological Information
Immunogen	Recombinant Protein
Epitope	N-Terminus
Host	Rabbit
Specificity	Cat. # AB2201 will recognize the N-terminus of mPER1.
Species Reactivity	Mouse Human Rat
Species Reactivity Note	Mouse, Human and Rat. May react with Drosophila, Arvicanthis niloticus and Mesocricetus auratus based on sequence homology. Reactivity with other species has not been tested.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	BAA94086
Gene Symbol	CAB2 KIAA0482 MGC88021 MGC9753 PER PERLD1 RIGUI hPER hPER1
Purification Method	Unpurified
UniProt Number	O35973
UniProt Summary	FUNCTION: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences CLOCK function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK\|NPAS2-BMAL1\|BMAL2-induced transactivation. SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity). INTERACTION: Q9R1X4:Timeless; NbExp=2; IntAct=EBI-1266764, EBI-1793117; SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. TISSUE SPECIFICITY: In brain, highest expression is observed in the SCN. Highly expressed in the pyramidal cell layer of the piriform cortex, the periventricular part of the caudate-putamen, many thalamic nuclei, and the granular layer of the cerebellar cortex. Weaker expression is detected in most area of the brain, including cortical and non cortical structures. Expression but no oscillations occurs in the glomerular and mitral cell layers of the olfactory bulb, the internal granular layer of the cerebellum, the cornu ammonis and dentate gyrus of the hyppocampus, the cerebral and piriform cortices. Also found in heart, brain, spleen, lung, liver, skeletal muscle, testis. Highest level in testis. Low level in kidney. DEVELOPMENTAL STAGE: Expressed in the suprachiasmatic nucleus (SCN) during late fetal and early neonatal life. INDUCTION: In the suprachiasmatic nucleus (SCN), behaves like a day-type oscillator, with maximum expression during the light period. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. In liver, peak levels at CT9. In the SCN, levels increase by light exposure during subjective night. Circadian oscillations also observed in skeletal muscle and liver but not in testis. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. PTM: Ubiquitinated. SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain. SIMILARITY: Contains 2 PAS (PER-ARNT-SIM) domains.
Molecular Weight	136 kDa

Biological Information

Immunogen

Recombinant Protein

Epitope

N-Terminus

Host

Rabbit

Specificity

Cat. # AB2201 will recognize the N-terminus of mPER1.

Species Reactivity

Mouse
Human
Rat

Species Reactivity Note

Mouse, Human and Rat. May react with Drosophila, Arvicanthis niloticus and Mesocricetus auratus based on sequence homology. Reactivity with other species has not been tested.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

BAA94086

Gene Symbol

CAB2
KIAA0482
MGC88021
MGC9753
PER
PERLD1
RIGUI
hPER
hPER1

Purification Method

Unpurified

UniProt Number

O35973

UniProt Summary

FUNCTION: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences CLOCK function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation.
SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity).
INTERACTION:
Q9R1X4:Timeless; NbExp=2; IntAct=EBI-1266764, EBI-1793117;

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
TISSUE SPECIFICITY: In brain, highest expression is observed in the SCN. Highly expressed in the pyramidal cell layer of the piriform cortex, the periventricular part of the caudate-putamen, many thalamic nuclei, and the granular layer of the cerebellar cortex. Weaker expression is detected in most area of the brain, including cortical and non cortical structures. Expression but no oscillations occurs in the glomerular and mitral cell layers of the olfactory bulb, the internal granular layer of the cerebellum, the cornu ammonis and dentate gyrus of the hyppocampus, the cerebral and piriform cortices. Also found in heart, brain, spleen, lung, liver, skeletal muscle, testis. Highest level in testis. Low level in kidney.
DEVELOPMENTAL STAGE: Expressed in the suprachiasmatic nucleus (SCN) during late fetal and early neonatal life.
INDUCTION: In the suprachiasmatic nucleus (SCN), behaves like a day-type oscillator, with maximum expression during the light period. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. In liver, peak levels at CT9. In the SCN, levels increase by light exposure during subjective night. Circadian oscillations also observed in skeletal muscle and liver but not in testis.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation.
PTM: Ubiquitinated.
SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain.
SIMILARITY: Contains 2 PAS (PER-ARNT-SIM) domains.

Molecular Weight

136 kDa

Product Usage Statements
Quality Assurance	Routinely tested by western blot on human brain lysate
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely tested by western blot on human brain lysate

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-mPER1 (residues 6-21) (rabbit polyclonal)

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询