Anti-monomethyl Histone H3 (Lys9), clone CMA306 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, PIA	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, PIA

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1248
Description	Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306
Alternate Names	H3K9me1 Histone H3 (mono methyl K9) H3-K9-HMTase 1 Histone H3-K9 methyltransferase 1 Lysine N-methyltransferase 1A Position-effect variegation 3-9 homolog Su(var)3-9 homolog 1 Suppressor of variegation 3-9 homolog 1 histone-lysine N-methyltransferase, H3 lysine-9 specific 1 suppressor of variegation 3-9 (Drosophila) homolog 1 suppressor of variegation 3-9 homolog 1 (Drosophila)
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Description

Catalogue Number

05-1248

Description

Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

Alternate Names

H3K9me1
Histone H3 (mono methyl K9)
H3-K9-HMTase 1
Histone H3-K9 methyltransferase 1
Lysine N-methyltransferase 1A
Position-effect variegation 3-9 homolog
Su(var)3-9 homolog 1
Suppressor of variegation 3-9 homolog 1
histone-lysine N-methyltransferase, H3 lysine-9 specific 1
suppressor of variegation 3-9 (Drosophila) homolog 1
suppressor of variegation 3-9 homolog 1 (Drosophila)

Background Information

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Product Information
Format	Purified
Control	Recombinant Histone H3 and HeLa acid extract.
Presentation	Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.

Product Information

Format

Purified

Control

Recombinant Histone H3 and HeLa acid extract.

Presentation

Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.

Applications
Application	Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.
Key Applications	Western Blotting Peptide Inhibition Assay
Application Notes	Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.

Applications

Application

Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.

Key Applications

Western Blotting
Peptide Inhibition Assay

Application Notes

Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.

Biological Information
Immunogen	KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.
Epitope	monomethylated H3 Lys9
Clone	CMA306
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.
Isotype	IgG2a
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003484
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol	EC 2.1.1.43 KMT1A MG44 OTTHUMP00000024298 SUV39H
Purification Method	Protein G Purified
UniProt Number	O43463
UniProt Summary	FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.7 ENZYME REGULATION: Inhibited by S-adenosyl-L-homocysteine. Ref.20 SUBUNIT STRUCTURE: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Ref.22 Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.24 Ref.25 SUBCELLULAR LOCATION: Nucleus. Centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.6 DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition. DOMAIN: Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. Ref.17 PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. Ref.6 Ref.21 Ref.26 Ref.27 SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 chromo domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain.
Molecular Weight	~17 kDa

Biological Information

Immunogen

KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.

Epitope

monomethylated H3 Lys9

Clone

CMA306

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.

Isotype

IgG2a

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_003484

Entrez Gene Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Gene Symbol

EC 2.1.1.43
KMT1A
MG44
OTTHUMP00000024298
SUV39H

Purification Method

Protein G Purified

UniProt Number

O43463

UniProt Summary

FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.7
ENZYME REGULATION: Inhibited by S-adenosyl-L-homocysteine. Ref.20
SUBUNIT STRUCTURE: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Ref.22 Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.24 Ref.25
SUBCELLULAR LOCATION: Nucleus. Centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.6
DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
DOMAIN: Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. Ref.17
PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. Ref.6 Ref.21 Ref.26 Ref.27
SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.

Molecular Weight

~17 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract. Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C for 1 year from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable at 2-8°C for 1 year from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-monomethyl Histone H3 (Lys9), clone CMA306 (mouse monoclonal)

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