Anti-IL-23R, cytoplasmic (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IHC, ELISA, FC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, IHC, ELISA, FC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1331
Description	Anti-IL-23R Antibody, cytoplasmic
Alternate Names	interleukin 23 receptor
Background Information	Interleukin 23 receptor (IL-23R) is a type I cytokine receptor that is a subunit of the receptor for IL23A/IL23. IL-23R pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. IL-23R associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3.

Description

Catalogue Number

06-1331

Description

Anti-IL-23R Antibody, cytoplasmic

Alternate Names

interleukin 23 receptor

Background Information

Interleukin 23 receptor (IL-23R) is a type I cytokine receptor that is a subunit of the receptor for IL23A/IL23. IL-23R pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. IL-23R associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3.

Product Information
Format	Affinity Purified
Control	Jurkat cell lysate
Presentation	Purified rabbit polyclonal in PBS with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Jurkat cell lysate

Presentation

Purified rabbit polyclonal in PBS with 0.05% sodium azide.

Applications
Application	Anti-IL-23R Antibody, cytoplasmic detects level of IL-23R & has been published & validated for use in WB, IH, ELISA, FC.
Key Applications	Western Blotting Immunohistochemistry ELISA Flow Cytometry
Application Notes	Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-23R in mouse spleen tissue. Flow Cytometry Analysis: 2-10 µg from a previous lot detected IL-23R in human PBMCs. Does not work in mouse for flow cytometry. ELISA: A previous lot of this antibody was tested on ELISA by an independent laboratory.

Applications

Application

Anti-IL-23R Antibody, cytoplasmic detects level of IL-23R & has been published & validated for use in WB, IH, ELISA, FC.

Key Applications

Western Blotting
Immunohistochemistry
ELISA
Flow Cytometry

Application Notes

Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-23R in mouse spleen tissue.

Flow Cytometry Analysis: 2-10 µg from a previous lot detected IL-23R in human PBMCs. Does not work in mouse for flow cytometry.

ELISA: A previous lot of this antibody was tested on ELISA by an independent laboratory.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human IL-23R at and around the cytoplasmic domain.
Epitope	Cytoplasmic Domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes the cytoplasmic domain of IL-23R. May detect other isoforms of IL-23R (see molecular weight).
Species Reactivity	Human Mouse
Species Reactivity Note	Proven to react with human and mouse. Does not work for mouse in flow cytometry.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_653302
Entrez Gene Summary	The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq]
Gene Symbol	il23r IBD17 IL-23R
Purification Method	Affinity Purfied
UniProt Number	Q5VWK5
UniProt Summary	FUNCTION: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. SUBUNIT STRUCTURE: Heterodimer with IL12RB1. In presence of IL23, the heterodimer forms the IL23 receptor. Interacts with JAK2 and in presence of IL23 with STAT3. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells. PTM: Phosphorylated in response to IL23. INVOLVEMENT IN DISEASE: Genetic variation in IL23R is associated with inflammatory bowel disease type 17 (IBD17) [MIM:612261]. Inflammatory bowel disease (IBD) is a form of remitting Crohn disease (CD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. CD is commonly classified as an autoimmune disease. Genetic variation in IL23R is associated with susceptibility to ulcerative colitis [MIM:191390]. It is a chronic inflammatory bowel disease. In ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers. Ulcerative colitis is commonly classified as autoimmune disease. Genetic variation in IL23R is associated with susceptibility to psoriasis [MIM:177900]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows and knees, and may be associated with severe arthritis. The lesions are caused by hyperproliferative keratinocytes and infiltration of inflammatory cells into the dermis and epidermis. The usual age of onset of psoriasis is between 15 and 30 years, although it can present at any age. SEQUENCE SIMILARITIES: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 2 fibronectin type-III domains.
Molecular Weight	~42 kDa Observed. May detect other isoforms of IL-23R produced by alternative splicing: isoform 1 (~72 kDa), isoform 2 (~43 kDa), isoform 3 (~41 kDa), isoform 4 (~20 kDa), isoform 5 (~30 kDa), isoform 6 (~43 kDa) and isoform 7 (~26 kDa).

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human IL-23R at and around the cytoplasmic domain.

Epitope

Cytoplasmic Domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes the cytoplasmic domain of IL-23R.
May detect other isoforms of IL-23R (see molecular weight).

Species Reactivity

Human
Mouse

Species Reactivity Note

Proven to react with human and mouse.
Does not work for mouse in flow cytometry.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_653302

Entrez Gene Summary

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq]

Gene Symbol

il23r
IBD17
IL-23R

Purification Method

Affinity Purfied

UniProt Number

Q5VWK5

UniProt Summary

FUNCTION: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

SUBUNIT STRUCTURE: Heterodimer with IL12RB1. In presence of IL23, the heterodimer forms the IL23 receptor. Interacts with JAK2 and in presence of IL23 with STAT3.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.

PTM: Phosphorylated in response to IL23.

INVOLVEMENT IN DISEASE: Genetic variation in IL23R is associated with inflammatory bowel disease type 17 (IBD17) [MIM:612261]. Inflammatory bowel disease (IBD) is a form of remitting Crohn disease (CD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. CD is commonly classified as an autoimmune disease.

Genetic variation in IL23R is associated with susceptibility to ulcerative colitis [MIM:191390]. It is a chronic inflammatory bowel disease. In ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers. Ulcerative colitis is commonly classified as autoimmune disease.

Genetic variation in IL23R is associated with susceptibility to psoriasis [MIM:177900]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows and knees, and may be associated with severe arthritis. The lesions are caused by hyperproliferative keratinocytes and infiltration of inflammatory cells into the dermis and epidermis. The usual age of onset of psoriasis is between 15 and 30 years, although it can present at any age.

SEQUENCE SIMILARITIES: Belongs to the type I cytokine receptor family. Type 2 subfamily.

Contains 2 fibronectin type-III domains.

Molecular Weight

~42 kDa Observed.
May detect other isoforms of IL-23R produced by alternative splicing: isoform 1 (~72 kDa), isoform 2 (~43 kDa), isoform 3 (~41 kDa), isoform 4 (~20 kDa), isoform 5 (~30 kDa), isoform 6 (~43 kDa) and isoform 7 (~26 kDa).

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected IL-23R on 10 µg of Jurkat cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-23R on 10 µg of Jurkat cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-IL-23R, cytoplasmic (rabbit polyclonal)

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