Anti-CD45RA, Clone MEM 56 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	FC, IF, IH(P), IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

FC, IF, IH(P), IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1413
Replaces	04-1102
Description	Anti-CD45RA Antibody, Clone MEM 56
Alternate Names	CD45 antigen Leukocyte common antigen precursor SCID due to PTPRC deficiency T200 glycoprotein T200 leukocyte common antigen Human homolog of severe combined immunodeficiency due to PTPRC deficiency Leukocyte-common antigen Protein tyrosine phosphatase, receptor type C Protein tyrosine phosphatase, receptor type C polypeptide
Background Information	CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Description

Catalogue Number

05-1413

Replaces

04-1102

Description

Anti-CD45RA Antibody, Clone MEM 56

Alternate Names

CD45 antigen
Leukocyte common antigen precursor
SCID due to PTPRC deficiency
T200 glycoprotein
T200 leukocyte common antigen
Human homolog of severe combined immunodeficiency due to PTPRC deficiency
Leukocyte-common antigen
Protein tyrosine phosphatase, receptor type C
Protein tyrosine phosphatase, receptor type C polypeptide

Background Information

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Product Information
Format	Purified
Control	RAW264 Cell Lysate
Presentation	Purified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Product Information

Format

Purified

Control

RAW264 Cell Lysate

Presentation

Purified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for use in its particular application.

Applications
Application	Anti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.
Key Applications	Flow Cytometry Immunofluorescence Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Immunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining. Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation. Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot. Immunohistochemistry(paraffin): Representative testing from a previous lot. Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue. Optimal Staining of CD45RA Monoclonal Antibody: Tonsil

Applications

Application

Anti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.

Key Applications

Flow Cytometry
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting

Application Notes

Immunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.

Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.

Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.

Optimal Staining of CD45RA Monoclonal Antibody: Tonsil

Biological Information
Clone	MEM 56
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems. Antigen distribution: B-cells (lg+) > 95% T-cells (E+) 70 + 10% Peripheral blood lymphocytes 68 + 8% T-cells (CD4+) 50 + 10% Monocytes 50 + 20% Thymocytes < 5% Granulocytes < 1%
Isotype	IgG2b
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Mouse. Expected to react with human and rat based on sequence homology. .
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002838.3
Entrez Gene Summary	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Gene Symbol	PTPRC L-CA LCA CD45 LY5 T200 Glycoprotein B220 GP180 EC 3.1.3.48
Purification Method	Purified
UniProt Number	P08575
UniProt Summary	FUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN. SIZE: 1304 amino acids; 147254 Da SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1. PTM: Heavily N- and O-glycosylated. DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
Molecular Weight	~220 kDa

Biological Information

Clone

MEM 56

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
Antigen distribution:
B-cells (lg+) > 95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes < 5%
Granulocytes < 1%

Isotype

IgG2b

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Mouse. Expected to react with human and rat based on sequence homology. .

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_002838.3

Entrez Gene Summary

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Gene Symbol

PTPRC
L-CA
LCA
CD45
LY5
T200
Glycoprotein
B220
GP180
EC 3.1.3.48

Purification Method

Purified

UniProt Number

P08575

UniProt Summary

FUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
SIZE: 1304 amino acids; 147254 Da
SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.

Molecular Weight

~220 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on RAW 264 lysates. Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on RAW 264 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	For use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

For use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CD45RA, Clone MEM 56 (mouse monoclonal)

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