Anti-phospho-EGFR (Tyr1069) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Po, Pm, Eq, R	ELISA, ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Po, Pm, Eq, R

ELISA, ICC, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-310
Replaces	04-339
Description	Anti-phospho-EGFR (Tyr1069) Antibody
Alternate Names	Receptor tyrosine-protein kinase ErbB-1 avian erythroblastic leukemia viral (v-erb-b) oncogene homolog cell growth inhibiting protein 40 cell proliferation-inducing protein 61 epidermal growth factor receptor epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Background Information	EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

Description

Catalogue Number

09-310

Replaces

04-339

Description

Anti-phospho-EGFR (Tyr1069) Antibody

Alternate Names

Receptor tyrosine-protein kinase ErbB-1
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
cell growth inhibiting protein 40
cell proliferation-inducing protein 61
epidermal growth factor receptor
epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Background Information

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

Product Information
Format	Affinity Purified
Control	Untreated and EGF treated A431 lysates
Presentation	Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Untreated and EGF treated A431 lysates

Presentation

Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.

Applications
Application	This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).
Key Applications	ELISA Immunocytochemistry Western Blotting
Application Notes	Immunofluroescent Immunocytochemistry:

Applications

Application

This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).

Key Applications

ELISA
Immunocytochemistry
Western Blotting

Application Notes

Immunofluroescent Immunocytochemistry:

Biological Information
Immunogen	Tyrosine-phosphorylated epidermal growth factor receptor.
Epitope	Tyr1069 (cytoplasmic domain)
Host	Rabbit
Specificity	Reacts strongly and specifically with Tyr1069 phosphorylated EGFR
Species Reactivity	Human Mouse Pig Primate Horse Rat
Species Reactivity Note	Tested in Human. Expected to react with mouse, rat, primate, horse and pig based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_005228.3
Entrez Gene Summary	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
Gene Symbol	ERBB ERBB1 HER1 PIG61 mENA EC 2.7.10.1
Modifications	Phosphorylation
Purification Method	Antigen Affinity Purified
UniProt Number	P00533
UniProt Summary	FUNCTION: Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Ref.32 Isoform 2/truncated isoform may act as an antagonist. Ref.32 Catalytic activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1. Ref.32 Ref.26 Ref.30 Ref.31 Ref.36 SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. TISSUE SPECIFICITY: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.39 PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.29 Ref.33 Ref.34 Ref.35 Ref.37 Ref.38 Ref.40 Ref.41 Ref.42 Ref.43 Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. DISEASE: Defects in EGFR are associated with lung cancer [MIM:211980]. Miscellaneous Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
Molecular Weight	~170 kDa

Biological Information

Immunogen

Tyrosine-phosphorylated epidermal growth factor receptor.

Epitope

Tyr1069 (cytoplasmic domain)

Host

Rabbit

Specificity

Reacts strongly and specifically with Tyr1069 phosphorylated EGFR

Species Reactivity

Human
Mouse
Pig
Primate
Horse
Rat

Species Reactivity Note

Tested in Human. Expected to react with mouse, rat, primate, horse and pig based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_005228.3

Entrez Gene Summary

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]

Gene Symbol

ERBB
ERBB1
HER1
PIG61
mENA
EC 2.7.10.1

Modifications

Phosphorylation

Purification Method

Antigen Affinity Purified

UniProt Number

P00533

UniProt Summary

FUNCTION: Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Ref.32

Isoform 2/truncated isoform may act as an antagonist. Ref.32

Catalytic activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1. Ref.32 Ref.26 Ref.30 Ref.31 Ref.36

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Secreted.

TISSUE SPECIFICITY: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.39

PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.29 Ref.33 Ref.34 Ref.35 Ref.37 Ref.38 Ref.40 Ref.41 Ref.42 Ref.43

Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

DISEASE: Defects in EGFR are associated with lung cancer [MIM:211980].

Miscellaneous Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.

SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Contains 1 protein kinase domain.

Molecular Weight

~170 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates. Western Blot Analysis: 1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-EGFR (Tyr1069) (rabbit polyclonal)

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