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Anti-Lin28 (rabbit polyclonal)

市场价：: 0.00

促销价：: ¥0.00

货号：07-1385

品牌：Millipore 密理博

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北京朝阳

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IP	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	07-1385
Description	Anti-Lin28 Antibody
Alternate Names	RNA-binding protein LIN-28
Background Information	Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

References

Product Information
Format	Serum
Presentation	Rabbit Serum

Applications
Application	Anti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation: Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.

Biological Information
Immunogen	Full-length His-tagged Lin28 recombinant protein (human).
Host	Rabbit
Specificity	Detects Lin28
Species Reactivity	Human Mouse
Species Reactivity Note	Human and Mouse. Other species have not been tested.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_078950
Gene Symbol	LIN-28 LIN28A
Purification Method	Unpurified
UniProt Number	Q9H9Z2
UniProt Summary	FUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity. SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity. SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity. SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. Ref.7 Ref.8 Ref.9 DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. Ref.7 Ref.8 Ref.9 INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Ref.6 Ref.10 DOMAIN: The CSD domain is required for function in muscle differentiation By similarity. SIMILARITY: Belongs to the lin-28 family. Contains 2 CCHC-type zinc fingers. Contains 1 CSD (cold-shock) domain.
Molecular Weight	25 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Western Blot Analysis: This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Packaging Information
Material Size	100 µL

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