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Anti-Actin, clone EP184E, Rabbit Monoclonal

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货号：04-1040

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, Ch, Eq, H, M, R IF, IHC, IH(P), WB M Purified Monoclonal Antibody
Description
Catalogue Number CBL171
Replaces 04-1040
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Actin Antibody, smooth muscle, clone ASM-1
Alternate Names
  • Alpha-actin-2
Background Information ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
References
Product Information
Format Purified
Control
  • Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Presentation Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Applications
Application Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.

Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
Epitope N-terminus
Clone ASM-1
Host Mouse
Specificity This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
Isotype IgG2a
Species Reactivity
  • Bovine
  • Chicken
  • Horse
  • Human
  • Mouse
  • Rat
Species Reactivity Note Human, mouse, and rat. Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
Gene Symbol
  • ACTA2
  • ACTSA
  • AAT6
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: P62736 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42009 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
SIMILARITY: SwissProt: P62736 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Molecular Weight 43 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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