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Anti-Vitronectin,clone EP873Y, Rabbit Monoclonal

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货号：04-1108

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MAB88917
Replaces 04-1108
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Vitronectin Antibody, clone 8E6(LJ8)
Alternate Names
  • Serum spreading factor
  • S-protein
References
Product Information
Format Purified
Presentation Purified immunoglobulin. Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Applications
Application Anti-Vitronectin Antibody, clone 8E6(LJ8) detects level of Vitronectin & has been published & validated for use in ELISA, WB, IH.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry
Application Notes Western blot: 5-20 μg/mL Stains cell-binding vitronectin.

Immunocytochemistry: 5-20 μg/mL

ELISA: 5-20 μg/mL

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Purified human vitronectin from plasma.
Clone 8E6(LJ8)
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity The antibody reacts with human vitronectin, it does not react with human fibronectin (Hayman et al., 1983).
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Gene Symbol
  • VTN
  • S-protein
  • epibolin
  • VNT
  • VN
  • V75
UniProt Number
UniProt Summary FUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
SIZE: 478 amino acids; 54306 Da
SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
SUBCELLULAR LOCATION: Secreted, extracellular space.
TISSUE SPECIFICITY: Plasma.
DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C in undiluted aliquots for up to 12 months.Avoid repeat freeze/thaw cycles.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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