Anti-phospho-MEK1/2 (Ser218/Ser222), clone E237, Rabbit Monoclonal

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Anti-phospho-MEK1/2 (Ser218/Ser222), clone E237, Rabbit Monoclonal

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货号：04-1017

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, IH(P), IP	Rb	Unpurified	Monoclonal Antibody

Description
Catalogue Number	04-1017
Description	Anti-phospho-MEK1/2 Ser218/Ser222) Antibody, clone E237, rabbit monoclonal
Alternate Names	MAP kinase kinase 1 MAPK/ERK kinase 1 MAPKK 1 mitogen-activated protein kinase kinase 1 protein kinase, mitogen-activated kinase 1 (MAP kinase kinase 1) ERK activator kinase 1
Background Information	MEK1/2, also known as MAP2K1, are mitogen activated protein kinases and are involved in cell growth and differentiation. MEK is activated by a variety of growth factors and cytokines resulting in phosphorylation at Ser217/218 and Ser221/222. Diseases associated with MEK1/2 is cardio-facio- cutaneous syndrome (CFC), which is an autosomal dominant trait.

References

Product Information
Format	Unpurified
Control	Untreated and EGF-treated serum-starved A431 cell lysate
Presentation	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Applications
Application	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications	Western Blotting Immunocytochemistry Immunohistochemistry (Paraffin) Immunoprecipitation
Application Notes	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected MEK1/2 in skin carcinoma tissue. Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:30 dilution from a previous lot was used in IP.

Biological Information
Immunogen	Phospho-synthetic peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1/2.
Epitope	Phosphorylated Ser218/Ser222
Clone	E237
Host	Rabbit
Specificity	The antibody only detects MEK1/2 phosphorylated on Ser218 and Ser222.
Isotype	IgG
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002755.2
Gene Symbol	EC 2.7.12.2 MAPKK1 MEK1 MKK1 PRKMK1
Modifications	Phosphorylation
Purification Method	Unpurified
UniProt Number	Q02750
UniProt Summary	FUNCTION: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by phosphorylation. SUBUNIT STRUCTURE: Interacts with MORG1 By similarity. Interacts with Yersinia yopJ. PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. INVOLVEMENT IN DISEASE: Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Molecular Weight	~45 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untreated and EGF-treated serum-starved A431 cell lysates. Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect MEK1/2 in untreated and EGF-treated serum-starved A431 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.