Anti-MDC1, clone P2B11 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, Chp, B	ICC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H, Chp, B

ICC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1572
Description	Anti-MDC1 Antibody, clone P2B11
Alternate Names	Nuclear factor with BRCT domains 1 homologue to Drosophila photoreceptor protein calphotin mediator of DNA damage checkpoint 1
Background Information	MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Description

Catalogue Number

05-1572

Description

Anti-MDC1 Antibody, clone P2B11

Alternate Names

Nuclear factor with BRCT domains 1
homologue to Drosophila photoreceptor protein calphotin
mediator of DNA damage checkpoint 1

Background Information

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Product Information
Format	Purified
Control	NIH/3T3 cells
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.

Product Information

Format

Purified

Control

NIH/3T3 cells

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.

Applications
Application	Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.
Key Applications	Immunocytochemistry Western Blotting
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells. Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

Applications

Application

Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.

Key Applications

Immunocytochemistry
Western Blotting

Application Notes

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.
Epitope	Unknown
Clone	P2B11
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes MDC1 at and around the N-terminus.
Isotype	IgG1κ
Species Reactivity	Mouse Human Chimpanzee Bovine
Species Reactivity Note	Demonstrated to react with mouse. Predicted to react with human, bovine (cow) and chimpanzee based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001010833.2
Entrez Gene Summary	The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq]
Gene Symbol	MDC1 NFBD1
Purification Method	Protein G Purified
UniProt Number	Q5PSV9
UniProt Summary	FUNCTION: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 By similarity. SUBUNIT STRUCTURE: Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with CHEK2/CHK2/CDS1, which requires ATM-mediated phosphorylation within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of G22P1/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8. Interacts with CEP164 By similarity. Interacts with H2AFX, which requires phosphorylation of H2AFX. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN. SUBCELLULAR LOCATION: Nucleus. Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AFX. Ref.1 DOMAIN: Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX By similarity. PTM: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint By similarity. SEQUENCE SIMILARITIES: Contains 2 BRCT domains. Contains 1 FHA domain.
Molecular Weight	~ 184 kDa. There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa.

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Epitope

Unknown

Clone

P2B11

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes MDC1 at and around the N-terminus.

Isotype

IgG1κ

Species Reactivity

Mouse
Human
Chimpanzee
Bovine

Species Reactivity Note

Demonstrated to react with mouse.
Predicted to react with human, bovine (cow) and chimpanzee based on 100% sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001010833.2

Entrez Gene Summary

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq]

Gene Symbol

MDC1
NFBD1

Purification Method

Protein G Purified

UniProt Number

Q5PSV9

UniProt Summary

FUNCTION: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 By similarity.

SUBUNIT STRUCTURE: Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with CHEK2/CHK2/CDS1, which requires ATM-mediated phosphorylation within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of G22P1/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8. Interacts with CEP164 By similarity. Interacts with H2AFX, which requires phosphorylation of H2AFX. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN.

SUBCELLULAR LOCATION: Nucleus. Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AFX. Ref.1

DOMAIN: Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX By similarity.

PTM: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint By similarity.

SEQUENCE SIMILARITIES: Contains 2 BRCT domains.

Contains 1 FHA domain.

Molecular Weight

~ 184 kDa. There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-MDC1, clone P2B11 (mouse monoclonal)

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