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Anti-RBMS1 (MSSP-1), clone 4D11 (mouse monoclonal)

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货号：05-1360

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number 05-1360
Description Anti-RBMS1(MSSP-1) Antibody, clone 4D11
Alternate Names
  • RNA binding motif
  • single stranded interacting protein 1
  • Single-stranded DNA-binding protein MSSP-1
  • Suppressor of CDC2 with RNA-binding motif 2
  • c-myc gene single strand binding protein 2
  • suppressor of cdc 2 (cdc13) with RNA binding motif 2
Background Information RNA-binding motif, single-stranded-interacting protein 1 (RBMS1 or MSSP-1) is a single-stranded DNA binding protein that is characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. At the developmental stage, RBMS1 (MSSP-1) is expressed at its highest levels during the G1 to S transition of the cell cycle and is most commonly found in placenta, lung and heart tissue. RBMS1 (MSSP-1) has been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis.
References
Product Information
Format Purified
Control
  • MCF-7 cell lysate
Presentation Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-RBMS1(MSSP-1) Antibody, clone 4D11 is a Mouse Monoclonal Antibody for detection of RBMS1(MSSP-1) & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A previous lot of this antibody was used in confocal fluorescent analysis of NIH/3T3 and A431 cells (See page 2).
Biological Information
Immunogen GST-tagged recombinant protein corresonding to RBMS1 (MSSP-1).
Clone 4D11
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody detects RBMS1 (MSSP-1).
Isotype IgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. Several transcript variants, resulting from alternative splicing and encoding different isoforms, have been described. A pseudogene for this locus is found on chromosome 12.
Gene Symbol
  • MGC15146
  • MGC3331
  • MSSP
  • MSSP-1
  • MSSP-2
  • MSSP-3
  • MSSP1
  • SCR2
  • YC1
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Single-stranded DNA binding protein that interacts with the region upstream of the C-myc gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Highest amounts are found in placenta, lung and heart.

DEVELOPMENTAL STAGE: Expressed at highest levels during the G1 to S transition of the cell cycle.

SEQUENCE SIMILARITIES: Contains 2 RRM (RNA recognition motif) domains.

SEQUENCE CAUTION: The sequence AAX93095.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA45923.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAA45923.1 differs from that shown. Reason: Frameshift at positions 319 and 360.

Molecular Weight ~50 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1-2 µg/mL of this antibody detected RBMS1 (MSSP-1) on 15 µg of MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable at 2-8°C for 1 year from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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