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Anti-ARF1, clone 3F1 (mouse monoclonal)

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货号：05-1427

品牌：Millipore 密理博

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Replacement Information
Replacement Information 05-1427 is a recommended replacement for MAB3779

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ELISA, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number 05-1427
Replaces MAB3779
Description Anti-ARF1 Antibody, clone 3F1
Alternate Names
  • ADP-ribosylation factor 1
Background Information ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
Applications
Application Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry (Paraffin)
Application Notes ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Biological Information
Immunogen Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
Epitope C-terminus
Clone 3F1
Host Mouse
Specificity Recognizes human ARF1
Isotype IgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • ARF1
Purification Method Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
SIZE: 181 amino acids; 20697 Da
SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21.
SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family
Molecular Weight ~21 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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