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Anti-Gb5 (rabbit polyclonal)

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号:07-1208

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information
Replacement Information 07-1208 is a recommended replacement for AB4068

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R, H WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-1208
Replaces AB4068
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Gβ5 Antibody
Background Information The Gβ5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa. It has also been found that Gβ5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gβ1-4.
References
Product Information
Format Purified
Control
  • L6 Cell Lysate, plasma membranes from rat cerebrum.
Presentation Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.
Applications
Application This Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.
Key Applications
  • Western Blotting
Application Notes Optimal working dilutions must be determined by end user.
Biological Information
Immunogen Unique peptide sequence derived from the D1 blade of the Gβ5 protein.
Epitope D1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes D1 area of Gβ5.
Isotype IgG
Species Reactivity
  • Mouse
  • Rat
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist.
Gene Symbol
  • GNB5
  • FLJ37457
  • FLJ43714
  • Gbeta5
  • GB5
Purification Method Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: O14775 # Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
SIZE: 395 amino acids; 43566 Da
SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP (By similarity).
TISSUE SPECIFICITY: Expressed in multiple tissues.
SIMILARITY: SwissProt: O14775 ## Belongs to the WD repeat G protein beta family. & Contains 7 WD repeats.
Molecular Weight This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G protein Gbeta5 on 10 μg of L6 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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