Anti-Bruton"s tyrosine kinase (Btk), clone 10D11 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, IP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

WB, IP

Purified

Monoclonal Antibody

Description
Catalogue Number	04-1578
Description	Anti-Bruton's tyrosine kinase (Btk) Antibody, clone 10D11
Alternate Names	Agammaglobulinaemia tyrosine kinase B-cell progenitor kinase Bruton agammaglobulinemia tyrosine kinase Bruton tyrosine kinase dominant-negative kinase-deficient Brutons tyrosine kinase tyrosine-protein kinase BTK
Background Information	Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).

Description

Catalogue Number

04-1578

Description

Anti-Bruton's tyrosine kinase (Btk) Antibody, clone 10D11

Alternate Names

Agammaglobulinaemia tyrosine kinase
B-cell progenitor kinase
Bruton agammaglobulinemia tyrosine kinase
Bruton tyrosine kinase
dominant-negative kinase-deficient Brutons tyrosine kinase
tyrosine-protein kinase BTK

Background Information

Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).

Product Information
Format	Purified
Control	Raji cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

Raji cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Detect Bruton's tyrosine kinase (Btk) using this Anti-Bruton's tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.

Applications

Application

Detect Bruton's tyrosine kinase (Btk) using this Anti-Bruton's tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.

Biological Information
Immunogen	GST-tagged recombinant protein corres-ponding to human Btk.
Epitope	Intracellular
Clone	clone 10D11
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes human Btk, MW ~77 kDa. A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.
Isotype	IgG1κ
Species Reactivity	Human Rat
Species Reactivity Note	Proven to react with human and rat.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000052.1
Entrez Gene Summary	Summary: The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement.
Gene Symbol	AGMX1 AT ATK BPK EC 2.7.10.2 IMD1 MGC126261 MGC126262 OTTHUMP00000023676 OTTHUMP00000063593 PSCTK1 XLA
Purification Method	Protein G Purified
UniProt Number	Q06187
UniProt Summary	FUNCTION: Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. Ref.12 Ref.15 CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. COFACTOR: Binds 1 zinc ion per subunit. ENZYME REGULATION: Inhibited by IBTK. Activated by phosphorylation. SUBUNIT STRUCTURE: Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain. Interacts with GTF2I and ARID3A. Ref.15 Ref.13 SUBCELLULAR LOCATION: Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Nucleus By similarity. PTM: Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein By similarity. INVOLVEMENT IN DISEASE: Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also called X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin. Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. Contains 1 Btk-type zinc finger. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Molecular Weight	~77 kDa

Biological Information

Immunogen

GST-tagged recombinant protein corres-ponding to human Btk.

Epitope

Intracellular

Clone

clone 10D11

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes human Btk, MW ~77 kDa.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.

Isotype

IgG1κ

Species Reactivity

Human
Rat

Species Reactivity Note

Proven to react with human and rat.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000052.1

Entrez Gene Summary

Summary: The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency
characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement.

Gene Symbol

AGMX1
AT
ATK
BPK
EC 2.7.10.2
IMD1
MGC126261
MGC126262
OTTHUMP00000023676
OTTHUMP00000063593
PSCTK1
XLA

Purification Method

Protein G Purified

UniProt Number

Q06187

UniProt Summary

FUNCTION: Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. Ref.12 Ref.15
CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
COFACTOR: Binds 1 zinc ion per subunit.
ENZYME REGULATION: Inhibited by IBTK. Activated by phosphorylation.
SUBUNIT STRUCTURE: Binds GTF2I through the PH domain. Interacts with SH3BP5 via the SH3 domain. Interacts with IBTK via its PH domain. Interacts with GTF2I and ARID3A. Ref.15 Ref.13
SUBCELLULAR LOCATION: Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Nucleus By similarity.
PTM: Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein By similarity.
INVOLVEMENT IN DISEASE: Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also called X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.

Molecular Weight

~77 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Raji cell lysate. Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Bruton"s tyrosine kinase (Btk), clone 10D11 (mouse monoclonal)

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