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Anti-Talin, C-terminus, clone TD77 (mouse monoclonal)

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货号：05-1144

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Ch, M WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number 05-1144
Replaces MAB3264
Description Anti-Talin Antibody, CT, clone TD77
Alternate Names
  • talin 1
Background Information Talin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
References
Product Information
Format Purified
Control
  • Jurkat cell lysate.
Presentation Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
Applications
Application Detect Talin using this Anti-Talin Antibody, C-terminus, clone TD77 validated for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.
Biological Information
Immunogen Human platelet protein
Epitope C-terminus
Clone TD77
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.
Isotype IgG1
Species Reactivity
  • Human
  • Chicken
  • Mouse
Species Reactivity Note Proven to react with human. Expected cross-reactivity in mouse and chicken.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ILWEQ
  • KIAA1027
  • TLN
Purification Method Purified from Protein A Sepharose™ chromatography
UniProt Number
UniProt Summary FUNCTION: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts By similarity.
SUBUNIT STRUCTURE: Binds with high affinity to vinculin and with low affinity to integrins. Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM.
SUBCELLULAR LOCATION: Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with LAYN at the membrane ruffles By similarity.
SEQUENCE SIMILARITIES: Contains 1 FERM domain.
Contains 1 I/LWEQ domain.
Molecular Weight 270 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain between 2 and 8°C for 1 year after date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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