Anti-Tubulin b-lll, clone TU-20, Alexa Fluor® 488 Conjugated

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, Pm, M, Po, R	ICC, IF, WB, IHC, IP	M	AlexaFluor®488	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, H, Pm, M, Po, R

ICC, IF, WB, IHC, IP

AlexaFluor®488

Monoclonal Antibody

Description
Catalogue Number	CBL412X
Description	Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated
Alternate Names	Tubulin beta-4 Tubulin beta-III tubulin, beta 3 tubulin, beta 4
Background Information	Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Description

Catalogue Number

CBL412X

Description

Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated

Alternate Names

Tubulin beta-4
Tubulin beta-III
tubulin, beta 3
tubulin, beta 4

Background Information

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Product Information
Format	AlexaFluor®488
Control	Rat Cortex Cells.
Presentation	Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide.

Product Information

Format

AlexaFluor®488

Control

Rat Cortex Cells.

Presentation

Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.05% sodium azide.

Applications
Application	Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated validated for use in IC, IF, WB, IH & IP.
Key Applications	Immunocytochemistry Immunofluorescence Western Blotting Immunohistochemistry Immunoprecipitation
Application Notes	Immunohistochemistry: 5-10 µg/mL on frozen brain tissue. Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications. Optimal working concentration must be determined by end user.

Applications

Application

Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®488 Conjugated validated for use in IC, IF, WB, IH & IP.

Key Applications

Immunocytochemistry
Immunofluorescence
Western Blotting
Immunohistochemistry
Immunoprecipitation

Application Notes

Immunohistochemistry: 5-10 µg/mL on frozen brain tissue.
Although this conjugated antibody will work for Western blotting and Immunoprecipitation, we recommend catalog number CBL412 if these are your primary applications.
Optimal working concentration must be determined by end user.

Biological Information
Epitope	C-terminus
Clone	TU-20
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
Isotype	IgG1
Species Reactivity	Bovine Human Primate Mouse Pig Rat
Species Reactivity Note	Reacts with Bovine, Human, Primate, Mouse, Porcine (Pig), and Rat. Reactivity with other species has not been determined.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001184110
Entrez Gene Summary	This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010].
Gene Symbol	MC1R TUBB4 beta-4
Purification Method	Protein G Purified
UniProt Number	Q13509
UniProt Summary	FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. SUBUNIT STRUCTURE: Dimer of alpha and beta chains. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. TISSUE SPECIFICITY: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. DOMAIN: The highly acidic C-terminal region may bind cations such as calcium. PTM: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules Probable. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. INVOLVEMENT IN DISEASE: Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. SEQUENCE SIMILARITIES: Belongs to the tubulin family.
Molecular Weight	50 kDa

Biological Information

Epitope

C-terminus

Clone

TU-20

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.

Isotype

IgG1

Species Reactivity

Bovine
Human
Primate
Mouse
Pig
Rat

Species Reactivity Note

Reacts with Bovine, Human, Primate, Mouse, Porcine (Pig), and Rat. Reactivity with other species has not been determined.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001184110

Entrez Gene Summary

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010].

Gene Symbol

MC1R
TUBB4
beta-4

Purification Method

Protein G Purified

UniProt Number

Q13509

UniProt Summary

FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
SUBUNIT STRUCTURE: Dimer of alpha and beta chains.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton.
TISSUE SPECIFICITY: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
DOMAIN: The highly acidic C-terminal region may bind cations such as calcium.
PTM: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules Probable.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
INVOLVEMENT IN DISEASE: Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
SEQUENCE SIMILARITIES: Belongs to the tubulin family.

Molecular Weight

50 kDa

Product Usage Statements
Quality Assurance	Routinely tested cortical neurons in Immunocytochemistry. Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely tested cortical neurons in Immunocytochemistry.
Immunocytochemical Analysis: 1:250 dilution of this lot detected β-III tubulin in rat cortex cell culture.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 6 months at 2-8°C from date of receipt.

Packaging Information
Material Size	40 µL

Packaging Information

Material Size

40 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Tubulin b-lll, clone TU-20, Alexa Fluor® 488 Conjugated

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询