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Anti-mouse Tks4 (rabbit polyclonal)

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货号：09-260

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, IP Rb Purified Polyclonal Antibody
Description
Catalogue Number 09-260
Description Anti-mouse Tks4 Antibody
Background Information Tks4 (gene symbol SH3PXD2B) is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, Tks4 may be involved in the recruitment of MT1-MMP and ECM degradation.
Tks4 is broadly expressed and localizes in the cytoplasm of the podosome.
References
Product Information
Format Purified
Control
  • NIH3T3 cell lysate.
Presentation Purified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.
Applications
Application Anti-mouse Tks4 Antibody is an antibody against mouse Tks4 for use in WB, IC & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Biological Information
Immunogen Recombinant protein containing amino acids 257-477 of mouse Tks4.
Epitope a. a. 257-477
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity The antibody recognizes mouse Tks4.
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Mouse and human (90% homology). Predicted to cross react with rat (96%), horse (88%), canine (87%) and bovine (87%) based on sequence homology. Reactivity with other species has not been tested.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: Binds a range of phosphoinositides including phosphatidylinositol 3,5-biphosphate and, to a lesser extent, phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol 5-biphosphate. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in the stromal-vascular fraction of white adipose tissue with moderate expression in heart, skeletal muscle and the mature adipocyte fraction of white adipose tissue. Expressed in adipocyte-differentiable cells but not in non-adipogenic cells.
DEVELOPMENTAL STAGE: Expression increases quickly after induction of adipocyte differentiation, reaches a maximum after 3 hours and decreases by 12 hours.
DOMAIN: The PX domain is required for binding phosphoinositides and can also bind to the third SH3 domain.
SEQUENCE SIMILARITES: Belongs to the SH3PXD2 family.
Contains 1 PX (phox homology) domain.
Contains 4 SH3 domains.
SEQUENCE CAUTION: The sequence BAC40843.1 differs from that shown. Reason: Frameshift at several positions.
Molecular Weight ~ 130 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated on a representative lot by Immunoprecipitation followed by Western blot on NIH3T3 cell lysate using Anti-mouse Tks4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at 2-8°C for up to 1 year from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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