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Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1)

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货号：05-337

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B, H IP, WB M Ascites Monoclonal Antibody
Description
Catalogue Number 05-337
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-MARCKS Antibody, clone 2F12
References
Product Information
Format Ascites
Presentation mouse ascites containing 0.05% sodium azide
Applications
Application Anti-MARCKS Antibody, clone 2F12 detects level of MARCKS & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Biological Information
Immunogen Purified recombinant human MARCKS; Epitope mapped to the carboxyl-terminal.
Clone 2F12
Host Mouse
Specificity MARCKS
Isotype IgG1
Species Reactivity
  • Bovine
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Gene Symbol
  • MARCKS
  • 80K-L
  • MACS
  • PRKCSL
  • FLJ14368
  • phosphomyristin
  • MRACKS
  • PKCSL
  • FLJ90045
Purification Method Ascites
UniProt Number
UniProt Summary FUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SIZE: 332 amino acids; 31555 Da
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
Molecular Weight none given
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on bovine brain extract
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 6 months at -20°C
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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