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Anti-TRA-1-60, clone TRA-1-60FITC conjugate

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货号：FCMAB115F

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC, ICC M FITC Monoclonal Antibody
Description
Catalogue Number FCMAB115F
Description Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate
Background Information Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
References
Product Information
Format FITC
Control
  • Pluripotent human embryonic stem (ES) cells
Presentation Purified mouse monoclonal IgM conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.








Immunocytochemical staining of fixed H9 human ES cells incubated for two hours at 2-8ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37ºC with 1:100 dilution of anti-TRA-1-60 FITC conjugated (Cat. No. FCMAB115F) monoclonal antibody. Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.Flow Cytometry:Antibody dilution for cellular staining:• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.• 5 µL for Guava Flow Cytometer• 10 µL for other Flow Cytometry instruments
Applications
Application Detect TRA-1-60 using this Anti-TRA-1-60 Antibody, clone TRA-1-60, FITC conjugate validated for use in FC & IC.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl


Biological Information
Immunogen - Whole Cells corresponding to the of TRA-1-60.
Clone TRA-1-60
Host Mouse
Specificity This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is seen with murine EC, EG, or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule
Isotype IgM
Species Reactivity
  • Human
Species Reactivity Note Tested and passed on Human. based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Gene Symbol
  • TRA-1-60
Purification Method Ammonium Sulfate Precipitation
Molecular Weight ~235/410 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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