RIPAb+™ Anti-SNRNP70

Species Reactivity	Key Applications
H, Ca, M, R, B	RIP, IP, WB

Species Reactivity

Key Applications

H, Ca, M, R, B

RIP, IP, WB

Description
Catalogue Number	03-103
Brand Family	Upstate
Trade Name	RIPAb+ Upstate
Description	RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set
Overview	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction. The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.

Description

Catalogue Number

03-103

Brand Family

Upstate

Trade Name

RIPAb+
Upstate

Description

RIPAb+ SNRNP70 - RIP Validated Antibody and Primer Set

Overview

RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.

Product Information
Format	Purified
Control	Included negative control rabbit IgG antibody and control primers specific for U1snRNA.
Presentation	Anti-SNRNP70 (Rabbit Polyclonal IgG) One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C. Normal Rabbit IgG One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, U1snRNA One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C. FOR: GGG AGA TAC CAT GAT CAC GAA GGT REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

Product Information

Format

Purified

Control

Included negative control rabbit IgG antibody and control primers specific for U1snRNA.

Presentation

Anti-SNRNP70 (Rabbit Polyclonal IgG)
One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C.

Normal Rabbit IgG
One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

RIP Primers, U1snRNA
One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.
FOR: GGG AGA TAC CAT GAT CAC GAA GGT
REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

Applications
Application	This RIPAb+ SNRNP70 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	RNA Binding Protein Immunoprecipitation (RIP) Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation from RIP lysate: RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).

Applications

Application

This RIPAb+ SNRNP70 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

Key Applications

RNA Binding Protein Immunoprecipitation (RIP)
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation from RIP lysate:
RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).

Biological Information
Immunogen	The SNRNP70 purified antibody is made against a synthetic peptide corresponding to a region of human SNRNP70.
Host	Rabbit
Specificity	SNRNP70
Species Reactivity	Human Canine Mouse Rat Bovine
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001009820
Gene Symbol	SNRNP70 U1SNRNP RNPU1Z RPU1 U1AP1 U1SNRNP70 SNRPN
Purification Method	Purified
UniProt Number	P08621
UniProt Summary	FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA. SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2. SUBCELLULAR LOCATION: Nucleus. POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked. Extensively phosphorylated on serine residues in the C-terminal region. INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus. SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight	SNRNP70, observed at ~60 & 48 kDa (Predicted molecular weight ~52 kDa).

Biological Information

Immunogen

The SNRNP70 purified antibody is made against a synthetic peptide corresponding to a region of human SNRNP70.

Host

Rabbit

Specificity

SNRNP70

Species Reactivity

Human
Canine
Mouse
Rat
Bovine

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_001009820

Gene Symbol

SNRNP70
U1SNRNP
RNPU1Z
RPU1
U1AP1
U1SNRNP70
SNRPN

Purification Method

Purified

UniProt Number

P08621

UniProt Summary

FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.

SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.

SUBCELLULAR LOCATION: Nucleus.

POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.

INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.

SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.

Molecular Weight

SNRNP70, observed at ~60 & 48 kDa (Predicted molecular weight ~52 kDa).

Product Usage Statements
Quality Assurance	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700). Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700).
Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	10 assays
Material Package	10 assays per set. Recommended use: ~5 μg antibody per RIP (dependent upon biological context).

Packaging Information

Material Size

10 assays

Material Package

10 assays per set. Recommended use: ~5 μg antibody per RIP (dependent upon biological context).

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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