Anti-Na+/K+ ATPase a-1,clone C464.6

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, Rb, M, Mk, Xn, Po, Ca, Sh	ICC, IHC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R, Rb, M, Mk, Xn, Po, Ca, Sh

ICC, IHC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-369
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6
Alternate Names	ATPase Na+/K+ transporting, alpha 3 polypeptide Na(+)/K(+) ATPase alpha(III) subunit Na(+)/K(+) ATPase alpha-3 subunit Na+/K+ -ATPase alpha 3 subunit Na+/K+ ATPase 3 Sodium pump subunit alpha-3 dystonia 12 sodium pump 3 sodium-potassium-ATPase alpha 3 polypeptide sodium/potassium-transporting ATPase alpha-3 chain
Background Information	Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume. In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.

Description

Catalogue Number

05-369

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

Alternate Names

ATPase
Na+/K+ transporting, alpha 3 polypeptide
Na(+)/K(+) ATPase alpha(III) subunit
Na(+)/K(+) ATPase alpha-3 subunit
Na+/K+ -ATPase alpha 3 subunit
Na+/K+ ATPase 3
Sodium pump subunit alpha-3
dystonia 12
sodium pump 3
sodium-potassium-ATPase
alpha 3 polypeptide
sodium/potassium-transporting ATPase alpha-3 chain

Background Information

Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.

Product Information
Format	Purified
Control	Alpha 1 mRNA is present in all cell types examined.
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at -20ºC. Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.

Product Information

Format

Purified

Control

Alpha 1 mRNA is present in all cell types examined.

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
-20ºC.
Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.

Applications
Application	Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
Key Applications	Immunocytochemistry Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations. Immunohistochemistry: 1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue. 1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended. 2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody. 3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.

Applications

Application

Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.

Key Applications

Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunoprecipitation:
2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.

Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.

1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.

Biological Information
Immunogen	Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Clone	C464.6
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
Isotype	IgG1κ
Species Reactivity	Human Rat Rabbit Mouse Monkey Xenopus Pig Canine Sheep
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001001586
Entrez Gene Summary	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
Gene Symbol	ATP1A3 MGC13276 RDP EC 3.6.3.9 DYT12
Purification Method	Protein G Chromatography
UniProt Number	P05023
UniProt Summary	FUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. SIZE: 1013 amino acids; 111749 Da SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability. SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
Molecular Weight	112 kDa

Biological Information

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Clone

C464.6

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.

Isotype

IgG1κ

Species Reactivity

Human
Rat
Rabbit
Mouse
Monkey
Xenopus
Pig
Canine
Sheep

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001001586

Entrez Gene Summary

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.

Gene Symbol

ATP1A3
MGC13276
RDP
EC 3.6.3.9
DYT12

Purification Method

Protein G Chromatography

UniProt Number

P05023

UniProt Summary

FUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
SIZE: 1013 amino acids; 111749 Da
SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

Molecular Weight

112 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated in western blot on rat brain microsomal preparations. Western Blot Analysis: 0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated in western blot on rat brain microsomal preparations.

Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Na+/K+ ATPase a-1,clone C464.6

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