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Anti-phospho-ACK1 (Tyr284)

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货号：09-142

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 09-142
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-ACK1 (Tyr284) Antibody
Alternate Names
  • TNK2
  • tyrosine kinase non-receptor 2
  • p21cdc42Hs
  • activated Cdc42-associated kinase 1
  • activated p21cdc42Hs kinase
References
Product Information
Format Affinity Purified
Control
  • Brain tissue
Presentation Immunoaffinity Purified rabbit polyclonal IgG in 0.02M PBS, 0.25M sodium chloride (pH 7.6) containing 0.1% sodium azide and 30% glycerol.
Applications
Application Detect phospho-ACK1 (Tyr284) using this Anti-phospho-ACK1 (Tyr284) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen KLH conjugated synthetic peptide corresponding to amino acids surrounding Tyr 284 of human ACK1.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes ACK1 phosphorylated on Tyrosine residue 284.
Isotype IgG
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Gene Symbol
  • TNK2
  • p21cdc42Hs
  • ACK1
  • ACK-1
  • FLJ45547
  • FLJ44758
  • ACK
Modifications
  • Phosphorylation
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q07912 # Downstream effector of CDC42 which mediates CDC42- dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
COFACTOR: Magnesium.
SIZE: 1036 amino acids; 114327 Da
SUBUNIT: Interacts with CDC42. Interacts with activated CSPG4.
SUBCELLULAR LOCATION: Cell membrane (By similarity).
SIMILARITY: SwissProt: Q07912 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 1 CRIB domain. & Contains 1 protein kinase domain. & Contains 1 SH3 domain.
Molecular Weight 114 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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