Anti-SAM 68

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IHC, ICC, IP	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, IHC, ICC, IP

Serum

Polyclonal Antibody

Description
Catalogue Number	07-415-I
Replaces	07-415
Description	Anti-Sam 68 Antibody
Alternate Names	KH domain-containing, RNA-binding, signal transduction-associated protein 1 GAP-associated tyrosine phosphoprotein p62 Src-associated in mitosis 68 kDa protein Sam68 p21 Ras GTPase-activating protein-associated p62 p68
Background Information	KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1,p68, p21 Ras GTPase-activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the “signal transduction and activation of RNA” (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice.

Description

Catalogue Number

07-415-I

Replaces

07-415

Description

Anti-Sam 68 Antibody

Alternate Names

KH domain-containing, RNA-binding, signal transduction-associated protein 1
GAP-associated tyrosine phosphoprotein p62
Src-associated in mitosis 68 kDa protein
Sam68
p21 Ras GTPase-activating protein-associated p62
p68

Background Information

KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1,p68, p21 Ras GTPase-activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the “signal transduction and activation of RNA” (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice.

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.

Product Information

Format

Serum

Presentation

Rabbit polyclonal serum with 0.05% sodium azide.

Applications
Application	This Anti-Sam 68 Antibody is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation for the detection of Sam 68 .
Key Applications	Western Blotting Immunohistochemistry Immunocytochemistry Immunoprecipitation
Application Notes	Western Blotting Analysis: 1:500-1:10,000 of a representative lot detected Sam 68 in RIPA lysates from HeLa cells. Immunohistochemistry Analysis: A 1:600 dilution from a representative lot detected Sam 68 in mouse colon tissue (Data courtesy of Stephane Richard, McGill University). Western Blotting Analysis: A representative lot detected Sam 68 in endogenous Sam 68 and exogenously expressed GFP-Sam 68 fusion in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033). Western Blotting Analysis: A representative lot detected Sam 68 in HEK293 transfected with GFP-Sam68, mouse cortex, hippocampus, cerebellum, and mouse brain (Iijima, T., et al. (2011). Cell. 147(7):1601-1614). Western Blotting Analysis: A representative lot detected Sam 68 in HeLa cells transfected with GFP-Sam68, MDA-MB-231, MDA-MB-468, and BT20 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647). Western Blotting Analysis: A representative lot detected Sam 68 in lysates from isolated mouse cells and mouse embryonic cell (Richard, S., et al. (2005). PLoS Genet. 1(6):e47). Immunohistochemistry Analysis: A representative lot detected Sam 68 in mouse cerebellum, parasagittal section of brain stem and cerebellum (Iijima, T., et al. (2011). Cell. 147(7):1601-1614). Immunohistochemistry Analysis: A representative lot detected Sam 68 in embryonic mice (Richard, S., et al. (2005). PLoS Genet. 1(6):e47). Immunocytochemistry Analysis: A representative lot detected Sam 68 in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033). Immunoprecipitation Analysis: A representative lot immunoprecipitated Sam 68 in HeLa, HeLa cells transfected with GFP-Sam68, and MDA-MB-468 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).

Applications

Application

This Anti-Sam 68 Antibody is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation for the detection of Sam 68 .

Key Applications

Western Blotting
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation

Application Notes

Western Blotting Analysis: 1:500-1:10,000 of a representative lot detected Sam 68 in RIPA lysates from HeLa cells.
Immunohistochemistry Analysis: A 1:600 dilution from a representative lot detected Sam 68 in mouse colon tissue (Data courtesy of Stephane Richard, McGill University).
Western Blotting Analysis: A representative lot detected Sam 68 in endogenous Sam 68 and exogenously expressed GFP-Sam 68 fusion in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Western Blotting Analysis: A representative lot detected Sam 68 in HEK293 transfected with GFP-Sam68, mouse cortex, hippocampus, cerebellum, and mouse brain (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Western Blotting Analysis: A representative lot detected Sam 68 in HeLa cells transfected with GFP-Sam68, MDA-MB-231, MDA-MB-468, and BT20 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).
Western Blotting Analysis: A representative lot detected Sam 68 in lysates from isolated mouse cells and mouse embryonic cell (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in mouse cerebellum, parasagittal section of brain stem and cerebellum (Iijima, T., et al. (2011). Cell. 147(7):1601-1614).
Immunohistochemistry Analysis: A representative lot detected Sam 68 in embryonic mice (Richard, S., et al. (2005). PLoS Genet. 1(6):e47).
Immunocytochemistry Analysis: A representative lot detected Sam 68 in HeLa cells (Chen, T., et al. (1999). Mol Biol Cell. 10(9):3015-3033).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sam 68 in HeLa, HeLa cells transfected with GFP-Sam68, and MDA-MB-468 (Lukong, K.E., et al. (2005). J Biol Chem. 280(46):38639-38647).

Biological Information
Immunogen	KLH-conjugated peptide corresponding to amino acid residues 331-348 of human Sam 68
Epitope	aa 331-348
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This antiserum (also known as AD-1 or AD1) recognizes Sam68, but not two other GSG (GRP33, Sam68, GLD-1) proteins, the Sam68-like mammalian proteins SLM-1 and SLM-2.
Species Reactivity	Human Mouse
Species Reactivity Note	Human, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_035447
Gene Symbol	Khdrbs1
Purification Method	Unpurified
UniProt Number	Q60749
Molecular Weight	~68 kDa observed

Biological Information

Immunogen

KLH-conjugated peptide corresponding to amino acid residues 331-348 of human Sam 68

Epitope

aa 331-348

Concentration

Please refer to lot specific datasheet.

Host

Rabbit

Specificity

This antiserum (also known as AD-1 or AD1) recognizes Sam68, but not two other GSG (GRP33, Sam68, GLD-1) proteins, the Sam68-like mammalian proteins SLM-1 and SLM-2.

Species Reactivity

Human
Mouse

Species Reactivity Note

Human, Mouse. Predicted to react with Rat based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_035447

Gene Symbol

Khdrbs1

Purification Method

Unpurified

UniProt Number

Q60749

Molecular Weight

~68 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MDA-MB-468 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Sam68 in 10 µg of MDA-MB-468 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in MDA-MB-468 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Sam68 in 10 µg of MDA-MB-468 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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