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Anti-PRMT1

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货号：07-404

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-404
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-PRMT1 Antibody
Background Information The methylation of arginine is mediated by the Protein Arginine Methyl Transferase (PRMT) family of enzymes. These enzymes transfer the methyl group from S-adenosyl-Lmethionine to the guanidino nitrogen atoms of an arginine residue. Arginine methylation was found to be an important modification in signal transduction, transcription, RNA transport, and splicing. PRMT1, also known as HRMT1L2 and IR1B4, was isolated through its interaction with BTG1 and TIS21, proteins that are important in cell quiescence. PRMT1 is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively). Proteins involved in the metabolism of RNA are substrates for PRMT1, among them Sam68.
References
Product Information
Format Purified
Control
  • Jurkat cell lysate
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Anti-PRMT1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of PRMT1 & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Peptide corresponding to residues 298-318 of human protein arginine methyltransferase 1.
Host Rabbit
Specificity PRMT1 doublet
Isotype IgG
Species Reactivity
  • Human
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM]
Gene Symbol
  • PRMT1
  • IR1B4
  • HCP1
  • HMT2
  • ANM1
  • HRMT1L2
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q99873 # Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones). Methylates SUPT5H.
SIZE: 361 amino acids; 41516 Da
SUBUNIT: Homodimer. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP (By similarity).
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: Q99873 ## Belongs to the protein arginine N-methyltransferase family.
Molecular Weight 42-44 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot in lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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