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Anti-B-Raf, NT

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货号：07-583

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R Enzyme Assays, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-583
Replaces 04-328
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-B-Raf Antibody, NT
References
Product Information
Format Affinity Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Anti-B-Raf Antibody, NT detects level of B-Raf & has been published & validated for use in EA & WB.
Key Applications
  • Enzyme Assays
  • Western Blotting
Biological Information
Immunogen synthetic peptide corresponding to amino acids 10-22 of human B-Raf
Host Rabbit
Specificity B-Raf
Isotype IgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • BRAF
  • B-raf-1
  • BRAF1
  • MGC126806
  • B-Raf
  • p94
  • MGC138284
  • RAFB1
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight 95kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on RIPA lysates from MCF-7 and PC12 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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