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Anti-Ago 2 (rabbit polyclonal IgG)

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货号：07-590

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 07-590
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Ago 2 Antibody
Background Information This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
References
Product Information
Format Purified
Control
  • HeLa cell lysate, HL-60 cell lysate
Presentation Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Applications
Application Anti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.
Isotype IgG
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
Gene Symbol
  • EIF2C2
  • AGO2
  • Q10
  • PPD
  • MGC3183
  • Argonaute-2
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9UKV8 # Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi) (By similarity).
SIZE: 851 amino acids; 96799 Da
SUBUNIT: Interacts with DICER1 through its Piwi domain.
SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Cytoplasmic bodies.
DOMAIN:SwissProt: Q9UKV8 Piwi domain is thought to provide RNA cleavage activity with a mechanism similar to RNase H (By similarity).
SIMILARITY: Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
Molecular Weight 100 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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