D8-MMAF hydrochloride 纯度:99.56% 100mg

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页酶、辅酶及抑制剂MCE>

图片仅供参考，请以实物为准

D8-MMAF hydrochloride 纯度:99.56% 100mg

市场价：: 0.00

促销价：: ¥0.00

货号：HY-15579AS

品牌：MCE

配送至：

北京朝阳

支付：账期货到付款

数量：

支 (预计4-5工作日到货(以MCE官网货期为准))

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

D8-MMAF hydrochloride是MMAF hydrochloride的氘代形式。

Description

D8-MMAF hydrochloride is a deuterated form of MMAF hydrochloride, which is a microtubule disrupting agent.

IC₅₀ & Target

IC50: 119 nM (Cytotoxicity, Karpas 299 cell), 105 nM (Cytotoxicity, H3396 cell), 257 nM (Cytotoxicity, 786-O cell), 200 nM (Cytotoxicity, Caki-1, cell)^[1]

In Vitro

MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC₅₀ values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF₄ is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested^[1].

In Vivo

The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF₄ has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF₄ ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively^[1].

Solvent & Solubility

In Vitro:

10 mM in DMSO

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2879 mL	6.4394 mL	12.8788 mL
5 mM	0.2576 mL	1.2879 mL	2.5758 mL
10 mM	0.1288 mL	0.6439 mL	1.2879 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Doronina SO, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24.

Cell Assay ^[1]	Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC₅₀ values is done using Alamar Blue dye reduction assay^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice: When subcutaneous Karpas 299 tumor size reaches 300 mm³, three animals per group receives one injection of 10 mg antibody component/kg body weight of either cAC10-L1-MMAF₄ or cBR96-L1-MMAF₄ intravenously. Tumors are then removed and placed in optimal cutting temperature compound, and 5 μm-thin frozen tissue sections are stained using immunohistochemistry evaluation^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Doronina SO, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24.

Molecular Weight

776.47

Formula

C₃₉H₅₈D₈ClN₅O₈

SMILES

CC[C@H](C)[C@@H]([C@@H](CC(N1[C@@H](CCC1)[C@@H]([C@@H](C)C(N[C@H](C(O)=O)CC2=CC=CC=C2)=O)OC)=O)OC)N(C([C@@](C([2H])(C([2H])([2H])[2H])C([2H])([2H])[2H])([2H])NC([C@H](C(C)C)NC)=O)=O)C.Cl

Storage